We narrowed to 4,028 results for: pCMV mammalian

1. pCMV6-G1-ATP6-Myc-FLAG (puromycin)

   Plasmid

   #86851

   Purpose

   mammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tags

   Depositor

   Matthew O'Connor

   Article

   Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

   Insert

   ATP6 (ATP6 Human)
   

   Use

   Tags

   ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

   Expression

   Mammalian

   Mutation

   codon corrected

   Promoter

   CMV

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-EGFP-eDHFR(69K6)-MCS

   Plasmid

   #172100

   Purpose

   Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   EGFP-eDHFR(69K6)-20aa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   eDHFR(69K6): Hexalysine (K6) sequence is inserted…

   Promoter

   CMV

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV HA hRB delta CDK + T373

   Plasmid

   #58912

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with T373 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-MCS-eDHFR(69K6)-EGFP

   Plasmid

   #172101

   Purpose

   Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   22aa-eDHFR(69K6)-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   eDHFR(69K6): Hexalysine (K6) sequence is inserted…

   Promoter

   CMV

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV HA hRB delta CDK + S612

   Plasmid

   #58914

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S612 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCMV HA hRB delta CDK + S608

   Plasmid

   #58913

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S608 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

   Plasmid

   #160140

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(nucleoplasmin)-3xHA-P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

   Plasmid

   #176458

   Purpose

   Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

   Plasmid

   #99229

   Purpose

   Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos2
   

   Use

   Tags

   Lifeact

   Expression

   Mammalian

   Mutation

   A69T for primed conversion applications

   Promoter

   CMV

   Available since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV6-G1-ATP8-Myc-FLAG (G418)

    Plasmid

    #86850

    Purpose

    mammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tags

    Depositor

    Matthew O'Connor

    Article

    Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

    Insert

    ATP8 (ATP8 Human)
    

    Use

    Tags

    ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG

    Plasmid

    #74261

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.Y591X truncation

    Promoter

    CMV

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pCMV HA hRB delta CDK + S780

    Plasmid

    #58915

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S780 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCMV HA hRB delta CDK + S811

    Plasmid

    #58919

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S811 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S811 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCMV HA hRB delta CDK + S807

    Plasmid

    #58918

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S807 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S807 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCMV HA hRB delta CDK + S788

    Plasmid

    #58916

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S788 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S788 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCMV HA hRB delta CDK + T356

    Plasmid

    #58911

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T356 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T356 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCMV HA hRB delta CDK + T821

    Plasmid

    #58920

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T821 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T821 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG

    Plasmid

    #74263

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.R404X truncation

    Promoter

    CMV

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCMV HA hRB delta CDK + T826

    Plasmid

    #58921

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T826 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T826 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCMV HA hRB delta CDK + S249

    Plasmid

    #58908

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S249 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S249 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only