21,184 results
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Plasmid#21334DepositorInsertDEPTOR (DEPTOR Human)
TagsFLAGExpressionMammalianMutationN204S and S389N mutations (numbering according to…Available SinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
Tet-on circRNA Expression Vector
Plasmid#92351PurposeThe vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.DepositorInsertmCherry and split EGFP
UseLentiviralExpressionMammalianPromoterpTetAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Talin head-SpyCatcher003
Plasmid#133566PurposeExpresses EGFP-Talin head-SpyCatcher003 in mammalian cellsDepositorInsertEGFP-Talin head-SpyCatcher003
ExpressionMammalianPromoterCMVAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE (AAV1)
Viral Prep#179459-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE (#179459). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE plasmid DNA. EF1a-driven, Cre-dependent soma and AIS-localized expression of voltage indicator JEDI-2P. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagscEGFP (Cre-dependent)Available SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1-GCaMP6m-XC
Plasmid#111543PurposeImproved genetic encoded calcium sensor based on GCaMP6m. Also called GCaMP-XDepositorInsertGCaMP6m-XC
ExpressionMammalianAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-mCherry (AAV9)
Viral Prep#114470-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-Ef1a-mCherry (#114470). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry plasmid DNA. EF1a-driven mCherry expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherryAvailable SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW2098
Plasmid#163094PurposeCRISPR repair template: add insertion site specific homology arms by PCR before insertionDepositorInsert30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
UseCRISPRExpressionWormMutationSilent mutations to remove piRNA sitesAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP (AAV8)
Viral Prep#137149-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP (#137149). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of Arch3.3-EYFP (inhibited in presence of Flp) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsEYFP (Cre-dependent)Available SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_CMV
Plasmid#99312PurposeLuciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertCMV enhancer
UseLuciferase; Starr-seq luciferase validation vectorExpressionMammalianAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyLigase
Plasmid#51722PurposePlasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!DepositorInsertSpyLigase
TagsHis6 TagExpressionBacterialPromoterT7Available SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only