We narrowed to 42,127 results for: Eras
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Plasmid#180436PurposeTransiently expressing a nicking sgRNA to facilitate the introduction of LRRK2-G2019S mutation in PE3 approachDepositorAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only
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pU6-pegRNA-SNCA-A53T
Plasmid#181738PurposeTransiently expressing a pegRNA to introduce SNCA-A53T mutation in human cellsDepositorInsertPrime editing pegRNA for SNCA-A53T (SNCA Synthetic)
UseCRISPRExpressionMammalianPromoterHuman U6Available SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBPK1520-SNCA-A53T-ng
Plasmid#181739PurposeTransiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approachDepositorInsertPrime editing ngRNA for SNCA-A53T (SNCA Human)
UseCRISPRExpressionMammalianPromoterHuman U6Available SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Promoter-hPE(+)-Luc
Plasmid#172003PurposeLuciferase Reporter for Human PTEN Enhancer PE - Forward OrientationDepositorInsertHuman PE sequence (hPE)
UseLuciferaseExpressionBacterial and MammalianPromoterSV40 PromoterAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Control click editor - pCMV-T7-PCV2-nCas9-deadEcKlenow (JO520)
Plasmid#208945PurposeA control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…PromoterCMV and T7Available SinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-pegRNA-SNCA-A30P
Plasmid#180016PurposeTransiently expressing a pegRNA to introduce SNCA-A30P mutation in human cellsDepositorInsertPrime editing pegRNA for SNCA-A30P (SNCA Synthetic)
UseCRISPRExpressionMammalianPromoterHuman U6Available SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBPK1520-SNCA-A30P-ng
Plasmid#180018PurposeTransiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approachDepositorInsertPrime editing ngRNA for SNCA-A30P (SNCA Human)
UseCRISPRExpressionMammalianPromoterHuman U6Available SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tre-Tight ifgMosaic Donor vector (LH121)
Plasmid#99630PurposeTo clone the 3 ORFs for Tet system (rtTA + Dox) dependent generation of ifgMosaicsDepositorInsertN-PhiM
UseCre/LoxMutation(Please see depositor comments below)Available SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ1A
Plasmid#197381PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-1 σ1A
TagsGAL4-DNA binding domain fragment, HA tag, and SV4…ExpressionYeastMutationContains an extra 42 nucleotides encoding 14 resi…PromoterADH1 and MET25Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-HA-Nr4a1-AA
Plasmid#160942PurposeGeneration of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)DepositorInsertNr4a1 (Nr4a1 Mouse)
UseRetroviralTagsHA tagExpressionMammalianMutationChange Cys 287 Glu 288 into Ala AlaPromoterMSCV-LTRAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only