We narrowed to 4,290 results for: AES

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pcDNA3.1_V5-TWIST1_TAL1ins

Plasmid

#215039

Purpose

Mammalian expression of human TWIST1 (with insertion from TAL1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop with insertion from TAL1 bHLH lo…

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1M

Plasmid

#215045

Purpose

Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Tags

V5

Expression

Mammalian

Mutation

Middle portion of TWIST1 bHLH loop (aa139, P) rep…

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_4XEboxMutant

Plasmid

#215026

Purpose

Firefly luciferase enhancer reporter plasmid with minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated

Use

Luciferase

Mutation

4X E-box mutations within Coordinator motifs

Promoter

SV40

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-DCK-Hygro

Plasmid

#202409

Purpose

gRNA expression vector for DCK

Depositor

Morito Kurata

Article

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

guide RNA (DCK Human)

Use

CRISPR and Lentiviral

Tags

Dck

Promoter

U6

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pIG-734pUC19-tNGFR-P2A-PIK3R1

Plasmid

#186107

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

PIK3R1 (PIK3R1 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-720pUC19-tNGFR-P2A-ZAP70

Plasmid

#186120

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

ZAP70 (ZAP70 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-735pUC19-tNGFR-P2A-PIK3CD

Plasmid

#186087

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

PIK3CD (PIK3CD Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-727pUC19-tNGFR-P2A-WASP

Plasmid

#186089

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

WASP (WAS Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-714pUC19-tNGFR-P2A-SAP

Plasmid

#186091

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

SAP (SH2D1A Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCri-H6-TEV-GRAB

Plasmid

#128498

Purpose

Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)

Tags

His tag (6x)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCri-H6-TEV-GRAB-STREP

Plasmid

#128499

Purpose

Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

GRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)

Tags

His tag (6x) and Strep tag (2x)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pIG-703pUC19-tNGFR-P2A-BACH2

Plasmid

#186112

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

BACH2 (BACH2 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-469 JUNB-tNGFR

Plasmid

#186060

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

JUNB (JUNB Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-472 FOXO1-tNGFR

Plasmid

#186062

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

FOXO1 (FOXO1 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-471 IRF4-tNGFR

Plasmid

#186061

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

IRF4 (IRF4 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-177 pUC19-CLTA-N-mCherry

Plasmid

#186128

Purpose

knockin of mCherry to CLTA target

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CLTA (CLTA Human)

Use

CRISPR

Mutation

WT with mCherry sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-374 tNGFR-P2A-B2M

Plasmid

#186068

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

B2M (B2M Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-STAT3mts

Plasmid

#195577

Purpose

Expresses STAT3 with mitochondrial targeting sequence

Depositor

Kevin Park

Article

Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

Insert

Signal transducer and activator of transcription 3 (Stat3 Mouse)

Use

AAV

Tags

Cox8 presequence

Expression

Mammalian

Promoter

CMV enhancer and promoter

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSOX9-dTAG-mNeonGreen-V5

Plasmid

#194971

Purpose

AAV vector for knocking in a C-terminal tag (dTAG-mNeonGreen-V5) for human SOX9

Depositor

Joanna Wysocka

Article

Naqvi et al Nat Genet. 2023 May;55(5):841-851. doi:

Insert

SRY-box transcription factor 9 (SOX9 Human)

Use

AAV

Tags

dTAG-mNeonGreen-V5

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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mAC-POLR2A donor (Hygro)

Plasmid

#124496

Purpose

A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover

Depositor

Masato Kanemaki

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)

Use

CRISPR

Tags

mAID-mClover

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGEN6-Ubc5 Sc

Plasmid

#83234

Purpose

pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Inserts

Ubiqutin (UBC Human)
Ubc 5 (UBC5 Budding Yeast)
Uba 1

Tags

His

Expression

Bacterial

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-EWSR1-gRNA

Plasmid

#237683

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to EWSR1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EWSR1 gRNA (EWSR1 Human)

Use

CRISPR

Tags

mCherry

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1A-WT-Flag-UTR

Plasmid

#183237

Purpose

Expression of FLAG tagged HNF1A

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox A (HNF1A Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-SS18-SSX2

Plasmid

#237637

Purpose

For overexpression of mEGFP-SS18-SSX2

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX2 (SS18, SSX2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pIG-747pUC19-tNGFR-P2A-CD7

Plasmid

#186086

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD7 (CD7 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-GIGYF1

Plasmid

#141188

Purpose

Expresses GFP-GIGYF1 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

GIGYF1 (GIGYF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pGL2 HNF4A P2 wt

Plasmid

#60324

Purpose

HNF4A P2 promoter cloned into the pGL2-basic backbone.

Depositor

Jorge Ferrer

Article

Hansen et al J Clin Invest. 2002 Sep;110(6):827-33.

Insert

NM_000457.4 (HNF4A Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
tet-pLKO.neo_shGFP

Plasmid

#110470

Purpose

Control, silence GFP gene, doxycycline inducible, neomycin selection

Depositor

Sandra Martha Gomes Dias

Article

Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-93

Insert

GFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1/TO (RNA PolIII)

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTR-338 pUC19-tNGFR-P2A-LAG3 N

Plasmid

#186055

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

LAG3 (LAG3 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4-His-Max-A-PQBP1-Y65C

Plasmid

#25803

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

Insert

PQBP1 with Y65C mutation (PQBP1 Human)

Tags

His and Xpress

Expression

Mammalian

Mutation

Tyrosine 65 within the WW domain of PQBP1 is chan…

Available Since

July 21, 2010

Availability

Academic Institutions and Nonprofits only