We narrowed to 15,401 results for: CCH

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pUDR418

Plasmid

#113875

Purpose

expression of double Cas9 programming sgRNA11 targetting STL1

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

doble sgRNA11-STL1

Use

CRISPR

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB8709

Plasmid

#175224

Purpose

Xenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

Depositor

Irina Borodina

Article

Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

Insert

YOL092W (CEN.PK strain)

Use

Mrna expression, injection into xenopus oocytes

Tags

PolyA

Promoter

T7

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB8709

Plasmid

#175224

Purpose

Xenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

Depositor

Irina Borodina

Article

Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

Insert

YOL092W (CEN.PK strain)

Use

Mrna expression, injection into xenopus oocytes

Tags

PolyA

Promoter

T7

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBY011-AMN1ko

Plasmid

#183099

Purpose

S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.

Depositor

Gabor Balazsi

Article

Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:

Insert

AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)

Tags

KanMX

Expression

Bacterial and Yeast

Mutation

only includes external sequences (504 bp each) as…

Promoter

URA3, TEF1

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBY011-AMN1ko

Plasmid

#183099

Purpose

S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.

Depositor

Gabor Balazsi

Article

Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:

Insert

AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)

Tags

KanMX

Expression

Bacterial and Yeast

Mutation

only includes external sequences (504 bp each) as…

Promoter

URA3, TEF1

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pB6-Lrig1-T2A-tdTomato-FNF-TK

Plasmid

#134320

Purpose

B6J Lrig1-T2A-tdTomato allele targeting vector, low copy number

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

Lrig1 (Lrig1 Mouse)

Use

Mouse Targeting

Tags

C-terminal fusion of T2A-tdTomato

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pB6-Cdk6-T2A-td-sfGFP-FNF-TK

Plasmid

#134321

Purpose

B6J Cdk6-T2A-td-sfGFP allele targeting vector, low copy number

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

Cdk6 (Cdk6 Mouse)

Use

Mouse Targeting

Tags

C-terminal fusion of T2A-tandem dimer-superfolder…

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pB6-Lrig1-T2A-tdTomato-FNF-TK

Plasmid

#134320

Purpose

B6J Lrig1-T2A-tdTomato allele targeting vector, low copy number

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

Lrig1 (Lrig1 Mouse)

Use

Mouse Targeting

Tags

C-terminal fusion of T2A-tdTomato

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pB6-Cdk6-T2A-td-sfGFP-FNF-TK

Plasmid

#134321

Purpose

B6J Cdk6-T2A-td-sfGFP allele targeting vector, low copy number

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

Cdk6 (Cdk6 Mouse)

Use

Mouse Targeting

Tags

C-terminal fusion of T2A-tandem dimer-superfolder…

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

Plasmid

#106462

Purpose

Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(N163,242Q)

Tags

PA

Expression

Bacterial and Yeast

Mutation

changed Asparagines at positions 163 and 242 to G…

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]

Plasmid

#106466

Purpose

Expresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(S219A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Serine 219 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]

Plasmid

#106467

Purpose

Expresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(H474A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Histidine 474 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]

Plasmid

#106468

Purpose

Expresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(D415A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Aspartate 415 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

Plasmid

#106462

Purpose

Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(N163,242Q)

Tags

PA

Expression

Bacterial and Yeast

Mutation

changed Asparagines at positions 163 and 242 to G…

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]

Plasmid

#106466

Purpose

Expresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(S219A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Serine 219 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]

Plasmid

#106467

Purpose

Expresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(H474A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Histidine 474 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]

Plasmid

#106468

Purpose

Expresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(D415A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Aspartate 415 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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p426_Cas9_gRNA-ARS208a

Plasmid

#87383

Purpose

All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS208a sequence GTCCGCTAAACAAAAGATCT in yeast chromosome 2.

Depositor

Aindrila Mukhopadhyay

Article

Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

Insert

Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS208a

Use

CRISPR

Promoter

ADH1, pTyrosine

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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p426_Cas9_gRNA-ARS208a

Plasmid

#87383

Purpose

All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS208a sequence GTCCGCTAAACAAAAGATCT in yeast chromosome 2.

Depositor

Aindrila Mukhopadhyay

Article

Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

Insert

Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS208a

Use

CRISPR

Promoter

ADH1, pTyrosine

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMEL12

Plasmid

#107918

Purpose

hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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