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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,224 results for: PAM

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Showing: 1821 - 1840 of 2224 results

  2. NSarQ27G-VMAn-FKBP

    Plasmid
    #70230
    Purpose
    Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (N-terminal portion)
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Gln 27 to Gly
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pcDNA3-NGFP-VMAn-FKBP

    Plasmid
    #70225
    Purpose
    Expresses N-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    eGFP
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Glu 125 to Ile, Deleted amino acids 129-2…
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. 3XFLAG-NSarQ27G-VMAn-FKBP

    Plasmid
    #70229
    Purpose
    Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (N-terminal portion)
    Tags
    3XFLAG, FK506- and rapamycin-binding protein (FKB…
    Expression
    Mammalian
    Mutation
    Changed Gln 27 to Gly
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pJK413

    Plasmid
    #155372
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    155372-plasmid-map-sequence-id-309000

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  20. pJK429

    Plasmid
    #155376
    Purpose
    PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    155376-plasmid-map-sequence-id-308997

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Showing: 1821 - 1840 of 2224 results