We narrowed to 4,936 results for: T2A

1. pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

   Plasmid

   #194925

   Purpose

   mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

   Depositor

   Hao Xu

   Article

   Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Promoter

   hPGK

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-AID-PcrA-UGI-T2A-mTagBFP2

   Plasmid

   #221224

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA-UGI-T2A-mTagBFP2
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-AID-PcrA-UGI-T2A-mTagBFP2

   Plasmid

   #221224

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA-UGI-T2A-mTagBFP2
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pC-attB-burs-mCD8-EGFP-T2A-Gal4

   Plasmid

   #39463

   Depositor

   Benjamin White

   Article

   Diao et al Genetics. 2012 Mar;190(3):1139-44. Epub

   Insert

   burs-mCD8-EGFP-T2A-Gal4
   

   Promoter

   bursalpha

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pC-attB-burs-mCD8-EGFP-T2A-Gal4

   Plasmid

   #39463

   Depositor

   Benjamin White

   Article

   Diao et al Genetics. 2012 Mar;190(3):1139-44. Epub

   Insert

   burs-mCD8-EGFP-T2A-Gal4
   

   Promoter

   bursalpha

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A

   Plasmid

   #191676

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A

   Depositor

   Veerle Baekelandt

   Inserts

   ATP10B (ATP10B Human)
   TMEM30A (TMEM30A Human)
   

   Use

   Lentiviral

   Tags

   6x HIS and flag

   Mutation

   D433N

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A

   Plasmid

   #191676

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A

   Depositor

   Veerle Baekelandt

   Inserts

   ATP10B (ATP10B Human)
   TMEM30A (TMEM30A Human)
   

   Use

   Lentiviral

   Tags

   6x HIS and flag

   Mutation

   D433N

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

    Plasmid

    #128749

    Purpose

    Expresses control gRNA

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

    Plasmid

    #128749

    Purpose

    Expresses control gRNA

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast

    Plasmid

    #205549

    Purpose

    Express EPN24-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast

    Plasmid

    #205549

    Purpose

    Express EPN24-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEpicVector3mC_Puro-T2A-mCherry-ProtC-FLAG-AU1

    Plasmid

    #162117

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to ProtC-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    ProtC-FLAG-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEpicVector3mC_Puro-T2A-mCherry-ProtC-FLAG-AU1

    Plasmid

    #162117

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to ProtC-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    ProtC-FLAG-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEpicVector3mC_Puro-T2A-mCherry-VSVg-FLAG-AU1

    Plasmid

    #162108

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to VSVg-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-FLAG-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEpicVector3mC_Puro-T2A-mCherry-VSVg-FLAG-AU1

    Plasmid

    #162108

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to VSVg-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-FLAG-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-FLAG

    Plasmid

    #162084

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-ProtC-FLAG
    

    Use

    Lentiviral

    Tags

    VSVg-ProtC-FLAG

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-AU1

    Plasmid

    #162091

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to StrepTagII-ProtC-AU1
    

    Use

    Lentiviral

    Tags

    StrepTagII-ProtC-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-FLAG

    Plasmid

    #162084

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-ProtC-FLAG
    

    Use

    Lentiviral

    Tags

    VSVg-ProtC-FLAG

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge