We narrowed to 24,871 results for: Spr

1. pGEL033

   Plasmid

   #137902

   Purpose

   STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing

   Depositor

   Yong Zhang

   Article

   Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-dCas9-CibN

   Plasmid

   #60552

   Purpose

   Expresses dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available Since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pspCas9-ABE-3'UTR-sgRNA-ST2-com vector

   Plasmid

   #136270

   Purpose

   Expresses ABE and ST2-com modified sgRNA scaffold

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Insert

   ABE and sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS433-pCSDest2-AcrIIC1Nme

   Plasmid

   #85679

   Purpose

   Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Nme from Neisseria meningitidis

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   Type II-C anti-CRISPR AcrIIC1Nme
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYC-Cas12g1-noncoding

   Plasmid

   #120880

   Purpose

   Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Noncoding sequences for CRISPR-Cas12g1
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbdCas9S(-10TC)_Psyn-sgRNA500

   Plasmid

   #149583

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30, Psyn

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYC-Cas12c2-noncoding

   Plasmid

   #120877

   Purpose

   Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Noncoding sequences for CRISPR-Cas12c2
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pV1464

   Plasmid

   #111440

   Purpose

   N. castellii Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISP_IS

   Plasmid

   #120425

   Purpose

   This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

   Depositor

   Tamás Fehér

   Article

   Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

   Insert

   CRISPR spacers targeting IS1, IS5, IS3
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   constitutive

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEG302 22aa SunTag NtDRMcd (no NLS) SUP

    Plasmid

    #115490

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBSR (mCherry-surrogateTarget-bsr)

    Plasmid

    #66950

    Purpose

    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    blasticidin
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Expression

    Mammalian

    Promoter

    beta-actin

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. px330-MRPsg1

    Plasmid

    #87189

    Purpose

    disruption of human RMRP gene via CRISPR-Cas9, guide 1

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    human MRP RNA CRISPR guide #1 (RMRP Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLsCas13aGG

    Plasmid

    #131012

    Purpose

    Backbone plasmid for generating CRISPR arrays for LsCas13a. Contains a GFP-dropout cassette and a direct repeat.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of LsCas13a
    GFP expression cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. px330-MRPsg2

    Plasmid

    #87190

    Purpose

    disruption of human RMRP gene via CRISPR-Cas9, guide 2

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    human MRP RNA CRISPR guide #2 (RMRP Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS436-pCSDest2-AcrIIC2Nme

    Plasmid

    #85712

    Purpose

    Mammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidis

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Type II-C anti-CRISPR AcrIIC2Nme
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

    Plasmid

    #85749

    Purpose

    Expresses anti-CRISPR protein from Type I-E and mTagBFP2

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Inserts

    Type I-E anti-CRISPR
    mTagBFP2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas12j2 VPR

    Plasmid

    #188518

    Purpose

    Expresses FLAG tagged dCas12j2 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D394A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSC44

    Plasmid

    #104818

    Purpose

    CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from AtUBQ10 promoter.

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.04g057400
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)

    Plasmid

    #178996

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

    Plasmid

    #178995

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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