We narrowed to 6,477 results for: sas

1. pJH3409

   Plasmid

   #179574

   Purpose

   Pceh-12::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans VB-MN -specific expression of miniSOG-SL2 RFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   wCherry

   Expression

   Worm

   Mutation

   Promoter

   Pceh-12

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTXB1-hMeCP2-G273X

   Plasmid

   #48093

   Purpose

   Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-G273X

   Depositor

   Huda Zoghbi

   Article

   Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

   Insert

   Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mxe intein/chitin binding domain tagged, expresse…

   Promoter

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

3. PLK3 1-334 T219D-pEGFP

   Plasmid

   #23268

   Depositor

   Wei Dai

   Article

   Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

   Insert

   PLK3 (PLK3 Human)
   

   Use

   Tags

   pEGFP

   Expression

   Mammalian

   Mutation

   aa 1-334 T219D

   Promoter

   Available since

   Dec. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

4. His 3C Kinase dead MKK6 1-334

   Plasmid

   #87882

   Purpose

   E. coli expression of MKK6 kinase dead

   Depositor

   Peter Mace

   Article

   Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Tags

   3C protease site and 6xHis

   Expression

   Bacterial

   Mutation

   K82A, kinase dead mutant

   Promoter

   T7

   Available since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FnoCas12a-DR-GC-swap-cr in pBCSK+

   Plasmid

   #126644

   Purpose

   Cloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnoCas12a Full Length Direct Repeat GC Swap crRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-f1(-)-ACTB-iTagRFP-T

   Plasmid

   #115916

   Purpose

   donor to insert iTagRFP-T at the ACTB locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   ACTB-TagRFP-T
   

   Use

   Donor template

   Tags

   TagRFP-T

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-f1(-)-SEC61B-dTomato

   Plasmid

   #115920

   Purpose

   donor to insert dTomato at the SEC61B locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   SEC61B-dTomato
   

   Use

   Donor template

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE MDproER

   Plasmid

   #13495

   Depositor

   Stephen Tapscott

   Article

   Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

   Insert

   MyoD1-Estrogen Receptor Fusion Protein (Myod1, ESR1 Mouse, Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   full length MyoD (containing the point mutation A…

   Promoter

   Available since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-ADAM7 (wt)

   Plasmid

   #31139

   Depositor

   Yardena Samuels

   Article

   Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

   Insert

   ADAM7 (ADAM7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pETDuet-msGC-NT21

    Plasmid

    #78102

    Purpose

    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

    Depositor

    William Montfort

    Article

    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.

    Inserts

    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    fragment 1-380 of the beta subunit and fragment 2…

    Promoter

    T7

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. myc-AP4 Epsilon (V454X)

    Plasmid

    #127624

    Purpose

    N-terminal myc-tagged AP4 epsilon subunit (V454X)

    Depositor

    Josef Kittler

    Article

    Ivankovic et al Autophagy. 2019 May 29:1-17. doi: 10.108

    Insert

    AP-4 complex subunit epsilon-1 (AP4E1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Premature stop codon (V454X)

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDF1-PTPRD-P459L

    Plasmid

    #25644

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

    Insert

    Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    tumor-derived mutation created by site-directed m…

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

13. PCRII apoEa

    Plasmid

    #121900

    Purpose

    Plasmid for making zebrafish apoEa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoEa (apoea Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221-hSOX10-Val15AlafsX11

    Plasmid

    #24732

    Depositor

    William Pavan

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    (sex determining region Y)-box 10 (SOX10 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    deleted amino acids 44-62. deletion/frameshift…

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pCMX-Gal4-hMeCP2-R270X-R111G

    Plasmid

    #48086

    Purpose

    Mammalian expression of Gal4 tagged MeCP2-R270X-R111G

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    R111G mutant MeCP2, expresses AT-hook R270X

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pETDuet-msGC-NT13

    Plasmid

    #75087

    Purpose

    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

    Depositor

    William Montfort

    Article

    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.

    Inserts

    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    fragment 1-380 of the beta subunit and fragment 4…

    Promoter

    Available since

    May 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBlue-hFX-HA

    Plasmid

    #14987

    Depositor

    Michael Ristow

    Article

    Shoichet et al Hum Mol Genet. 2002 Apr 1. 11(7):815-21.

    Insert

    human frataxin cDNA (FXN Human)
    

    Use

    Tags

    AU1 and HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pCDF1-PTPRD-D1248N

    Plasmid

    #25646

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

    Insert

    Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    tumor-derived mutation created by site-directed m…

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pET-22HT-MSI1(7-192)R53E/R59E/R61E

    Plasmid

    #60355

    Purpose

    R53E R59E R61E triple mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E, R59E and R61E mutations; contains amino aci…

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pNUTN6His Y426F/Y517F hTFNG

    Plasmid

    #70084

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobe

    Depositor

    Anne Mason, Ross MacGillivray

    Article

    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

    Insert

    mutated human serum transferrin (TF Human)
    

    Use

    Tags

    Hexa His tag and N-terminal signal peptide, 4 aa …

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp, Ty426Phe, Tyr517Phe

    Promoter

    SV40

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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