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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,217 results for: sgRNA

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Showing: 1841 - 1860 of 9217 results

  2. pDest_U6_A2_CG2_T1snp_gRNA

    Plasmid
    #213789
    Purpose
    pDEL114; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I (SNP) sgRNA
    Depositor
    William Talbot
    Insert
    nrg1 type I SNP sgRNA (nrg1 Zebrafish)
    Use
    Tol2 destination vector
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    213789-plasmid-map-sequence-id-421038

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  3. pCMV-BFP-U6-sgRNA

    Plasmid
    #196725
    Purpose
    Sortable sgRNA expression, Chen tracrRNA PMID: 24360272
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196725-plasmid-map-sequence-id-388067

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  4. sgRNA.SFFV.GFP

    Plasmid
    #169938
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and GFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.
    Depositor
    Benjamin Ebert
    Type
    Empty backbone
    Use
    Lentiviral
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    169938-plasmid-map-sequence-id-338362

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  6. peSCBE3-NG-HF1

    Plasmid
    #218164
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218164-plasmid-map-sequence-id-433171

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  7. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

    Plasmid
    #136656
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:MTH17CL396 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    136656-plasmid-map-sequence-id-275491

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  8. U6_sgRNA_CAG_hSt1Cas9_TH1477

    Plasmid
    #136655
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:TH1477 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136655-plasmid-map-sequence-id-273341

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  9. pFH102

    Plasmid
    #128207
    Purpose
    sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
    Depositor
    Vladimir Nekrasov
    Publication
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128207-plasmid-map-sequence-id-252909

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  14. Mammalian_SaAID3S

    Plasmid
    #188651
    Purpose
    Expresses SaAID3S and gRNA in mammalian cells
    Depositor
    Keiji Nishida
    Publication
    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    nSaCas9-AID3S--UGI
    Sa-sgRNA
    Tags
    UGI
    Expression
    Mammalian
    Mutation
    D10A for SaCas9
    Promoter
    EF-1a and H1
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188651-plasmid-map-sequence-id-375883

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  15. Mammalian_SaAID2S

    Plasmid
    #188652
    Purpose
    Expresses SaAID2S and gRNA in mammalian cells
    Depositor
    Keiji Nishida
    Publication
    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    AID2S-nSaCas9--UGI
    Sa-sgRNA
    Tags
    UGI
    Expression
    Mammalian
    Mutation
    D10A for SaCas9
    Promoter
    EF-1a and H1
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188652-plasmid-map-sequence-id-375885

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  16. MTK0_001

    Plasmid
    #123924
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TU-sgRNA - L1/RE
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123924-plasmid-map-sequence-id-250078

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Showing: 1841 - 1860 of 9217 results