We narrowed to 158,615 results for: Des

1. pDEST-FRT/T0-Flag-BRCA1

   Plasmid

   #71117

   Purpose

   mammalian expression of Flag-BRCA1 siRNA resistant construct

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV/TO

   Available Since

   Nov. 12, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pcDNA3.2-V5-DEST-hSFRP1-his

   Plasmid

   #189540

   Purpose

   Protein expression of SFRP1 in mammalian cells.

   Depositor

   Haian Fu

   Insert

   secreted frizzled related protein 1 (SFRP1 Human)
   

   Tags

   his

   Expression

   Mammalian

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDESTN-MTp-p53-Venus

   Plasmid

   #53736

   Purpose

   Expresses Venus tagged p53 under the metallothionein promoter

   Depositor

   Galit Lahav

   Article

   Batchelor et al Mol Cell. 2008 May 9;30(3):277-89. doi:

   Insert

   p53 (TP53 Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   metallothionein promoter

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDEST40-2XFL-Src-Y530F

   Plasmid

   #140319

   Purpose

   Expresses Flag-tagged human Src-Y530F in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   Y530F

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti CMV/TO DEST (775-1)

   Plasmid

   #17432

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PB-Hyg-Dest-mCherry-PAR-6B

   Plasmid

   #194908

   Purpose

   To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

   Depositor

   Clare Buckley

   Article

   Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

   Insert

   PAR-6B (Pard6b Mouse)
   

   Use

   Mouse Targeting

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   39 C to G, silent mutation

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LC201: pMVP/PB/Puro-DEST

   Plasmid

   #121874

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

   Plasmid

   #229828

   Purpose

   A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti X2 Puro DEST (w16-1)

   Plasmid

   #17296

   Purpose

   3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Puro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral and RNAi; Destination vector

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDestTol2CG2-mnx1:ReaChR-citrine

    Plasmid

    #169046

    Purpose

    Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    ReaChR-Citrine
    

    Tags

    Citrine

    Expression

    Mammalian

    Promoter

    3xMnx1

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid

    #37184

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Tags

    FokI nuclease

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Omega Destination-CMV::ELuc:bGH

    Plasmid

    #124528

    Purpose

    Omega destination vector, that has a CMV:ELUC:bgH in the backbone

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Type

    Empty backbone

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFRT/TO/FLAG/HA-DEST TNRC6A

    Plasmid

    #19883

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Trinucleotide repeat containing 6A (TNRC6A Human)
    

    Tags

    FLAG/HA

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDest-667 (attR4-attR3)

    Plasmid

    #161886

    Purpose

    Multisite Gateway (attR4-attR3) vector for lentivirus production

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQCXP CMV/TO DEST (w360-1)

    Plasmid

    #17386

    Purpose

    Retroviral Gateway destination vector, Expression, CMV/TO promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Retroviral; Destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST-mCherry-GJA1-20k-N1

    Plasmid

    #49861

    Purpose

    Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    GJA1-20k (GJA1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    GJA1-20k corresponds to residues 213 to 382 to Co…

    Promoter

    CMV

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFRT/TO/FLAG/HA-DEST TNRC6B

    Plasmid

    #19884

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    trinucleotide repeat containing 6B (TNRC6B Human)
    

    Tags

    FLAG/HA

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA 3.2/V5-DEST SNAP29-V5

    Plasmid

    #69821

    Purpose

    Expresses SNAP29-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Synaptosomal-associated protein 29 (SNAP29 Human)
    

    Use

    Retroviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST-mCherry-LacR-BRCA1

    Plasmid

    #71115

    Purpose

    mammalian expression of mCherry-LacR-BRCA1 siRNA resistant construct

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV/TO

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 362 pCS Cherry DEST

    Plasmid

    #13075

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    Cherry
    

    Available Since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge