We narrowed to 7,661 results for: Ski

1. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

   Plasmid

   #113687

   Purpose

   SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9 (NEWENTRY )
   

   Use

   AAV and CRISPR

   Tags

   Flag, HAx2, KASH, and NLS

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA

   Plasmid

   #113688

   Purpose

   SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9 (NEWENTRY )
   

   Use

   AAV and CRISPR

   Tags

   Flag, HAx2, KASH, and NLS

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV Cdk2ap1CAN-HA

   Plasmid

   #178030

   Purpose

   Retroviral vector for the purpose of overexpression in mammalian cell culture

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   First N-Terminal 27aa are deleted

   Promoter

   GAG

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-Blast POLE1 C-ISCmut

   Plasmid

   #160804

   Purpose

   Express C-terminal ISC mutant POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLE1 C-ISCmut (POLE Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Mutation

   shPOLE_1 and shPOLE2 resistant, C2221S, C2224S, C…

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA

   Plasmid

   #113689

   Purpose

   SaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9 (NEWENTRY )
   

   Use

   AAV and CRISPR

   Tags

   Flag, HAx2, KASH, and NLS

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

   Plasmid

   #51249

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 980,1006 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pQC MCS IRES G418

   Plasmid

   #110344

   Purpose

   γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Geneticin selection.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

   Plasmid

   #51247

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 849,865,877,907 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pMXS-IRES-Blast POLD1 ISCmut

   Plasmid

   #160806

   Purpose

   Express ISC mutant POLD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLD1 ISCmut (POLD1 Human)
   

   Use

   Retroviral

   Mutation

   Codon optimized C-terminal 230 amino acids, C1058…

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-His6-SUMO-Lyn-SH2-AviTag

    Plasmid

    #214207

    Purpose

    Bacterial expression of SH2 domain of the Lyn kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    Lyn A (LYN Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-His6-TEV-Grb2-SH2-mCherry-AviTag

    Plasmid

    #214231

    Purpose

    Bacterial expression of SH2 domain of the Grb2 with N-terminal TEV-cleavable 6xHis tag, C-terminal mCherry, and C-terminal AviTag for Streptavidin bead functionalization for binder selections.

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    Grb2 SH2 domain (GRB2 Human)
    

    Tags

    6xHis, AviTag, and mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-His6-SUMO-Lck-SH2-AviTag

    Plasmid

    #214203

    Purpose

    Bacterial expression of SH2 domain of the Lck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    Lck kinase SH2 domain (LCK Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28-His6-SUMO-Blk-SH2-AviTag

    Plasmid

    #214204

    Purpose

    Bacterial expression of SH2 domain of the Blk kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    Blk kinase SH2 domain (BLK Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28-His6-SUMO-Hck-SH2-AviTag

    Plasmid

    #214206

    Purpose

    Bacterial expression of SH2 domain of the Hck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    p60-HCK (HCK Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. PAX3::FOXO1-2A-sfGFP

    Plasmid

    #240098

    Purpose

    human PAX3::FOXO1 in -2A-sfGFP backbone (Plasmid# 74668)

    Depositor

    Genevieve Kendall

    Article

    Kucinski et al Cell Rep. 2025 Jul 22;44(7):115923. doi:

    Insert

    PAX3::FOXO1 (FOXO1, PAX3 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW-TAZ-CAMTA1

    Plasmid

    #235681

    Purpose

    Express TAZ-CAMTA1 fusion gene in mammalian cells using the doxycycline-inducible TRE promoter

    Depositor

    Duojia Pan

    Article

    Driskill et al Genes Dev. 2021 Apr 1;35(7-8):495-511. d

    Insert

    TAZ-CAMTA1 (WWTR1, CAMTA1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA

    Plasmid

    #233046

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and hfCas13d and mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA

    Plasmid

    #233045

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. LENTICRISPR-GPI_sgRNA2

    Plasmid

    #201595

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    GPI (GPI Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro

    Plasmid

    #201677

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette

    Depositor

    Timo Otonkoski

    Article

    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

    Insert

    MIR302-3g-EEA-2g-PGK-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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