We narrowed to 5,570 results for: donor

1. pGT-Kh2

   Plasmid

   #122587

   Purpose

   MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-SEP-GluA2 TKIT

   Plasmid

   #169442

   Purpose

   Expression of 2 guides + donor DNA

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   AAV and CRISPR

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

   Plasmid

   #174025

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   eGFP
   

   Use

   Zebrafish knock-in tagging

   Mutation

   Y40N

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AICSDP-19:LAMP1-mEGFP

   Plasmid

   #101782

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1

   Depositor

   Allen Institute for Cell Science

   Insert

   LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. AICSDP-42:AAVS1-mTagRFPT-CAAX

   Plasmid

   #107580

   Purpose

   Homology arms, promoter and linker-mTagRFPT-CAAX sequence for internal insertion of CAGGS-driven mTagRFPT-CAAX at the AAVS1 safe harbor location in human cells

   Depositor

   Allen Institute for Cell Science

   Insert

   AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   May 9, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAAV-SEP-GluA1 TKIT

   Plasmid

   #169441

   Purpose

   Expression of 2 guides + donor DNA

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   AAV and CRISPR

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AICSDP-116:PCNA-mEGFP

   Plasmid

   #170818

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA

   Depositor

   Allen Institute for Cell Science

   Insert

   PCNA Homology Arms with mEGFP-linker (PCNA Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   June 11, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pGT-Ah1

   Plasmid

   #122568

   Purpose

   MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA

   Plasmid

   #174029

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-mScarlet
   

   Use

   Zebrafish knock-in tagging

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. AICSDP-079:DCP1A-mEGFP

    Plasmid

    #193922

    Purpose

    Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A

    Depositor

    Allen Institute for Cell Science

    Insert

    DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Dec. 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. LT1_36_QUEEN-2m

    Plasmid

    #177708

    Purpose

    Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    pTEF1-QUEEN2m
    

    Use

    Used as donor dna in genome integration after lin…

    Expression

    Yeast

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPaint-2A-TurboGFP-PEST

    Plasmid

    #67180

    Purpose

    CRISPaint universal donor for gene expression quantification using destabilized TurboGFP

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    T2A-TurboGFP-PEST
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Halo-53BP1 HRD

    Plasmid

    #207087

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pR26-CAG-H2B-miRFP703

    Plasmid

    #112853

    Purpose

    Donor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell lines

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    H2B-miRFP703
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLHDR01_mCherry_tCD19

    Plasmid

    #123661

    Purpose

    Construction of 1st donor for scarless genome editing

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Type

    Empty backbone

    Use

    Mouse Targeting; Human targeting

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AICSDP-166:CDH1-mEGFP

    Plasmid

    #193921

    Purpose

    Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1

    Depositor

    Allen Institute for Cell Science

    Insert

    cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
    

    Use

    AAV and CRISPR; Donor template

    Tags

    linker-mEGFP

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Dec. 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. LT2_7_OxPro

    Plasmid

    #177709

    Purpose

    Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTRX2_5xUAS-ymYPET-tCYC1
    pTEFmut8-mCherry-tADH1
    

    Use

    Used as donor dna in genome integration after lin…

    Expression

    Yeast

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1_tdTomato

    Plasmid

    #198041

    Purpose

    Bxb1-specific donor plasmid for integrating a transcription unit for constitutive expression of tdTomato reporter.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

    Insert

    tdTomato
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AICSDP-60: SOX2-mEGFP

    Plasmid

    #124606

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SOX2

    Depositor

    Allen Institute for Cell Science

    Insert

    SOX2 Homology Arms with linker-mEGFP (SOX2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    May 13, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pUC19-TgKI-MCS-eGFP-MCS-polyA

    Plasmid

    #174024

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    eGFP
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge