We narrowed to 32,235 results for: uros

1. pLV(shRNA)-Puro-U6-CCEPR

   Plasmid

   #108277

   Purpose

   shRNA for CCEPR lncRNA

   Depositor

   Karl Munger

   Article

   Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

   Insert

   CCEPR (CCEPR Human)
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Myog'-2A-mCherryNLS-PGK-Puro

   Plasmid

   #69548

   Purpose

   Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Inserts

   2A-mCherry-NLS
   Myogenin 5' homology arm
   Myogenin 3' homology arm (Myog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2x nuclear localization signal and PVT1-2A "…

   Mutation

   Silent V205V mutation in myogenin gene to prevent…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-puro HA-Atg14*dCC

   Plasmid

   #38266

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

   Insert

   autophagy related genes 14 (ATG14 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   changed A618T, A621T, C624A and T627C for siRNA r…

   Promoter

   LTR

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W

   Plasmid

   #105037

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nprl2 (Nprl2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W

   Plasmid

   #105038

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nprl2 (Nprl2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pALPS puro miR30-STAT1

   Plasmid

   #117160

   Purpose

   Target site ATATCCAGTTCCTTTAGGGCC

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   shRNA targeting miR30
   

   Use

   Lentiviral

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE-puro-mTurquoise2-Nuf2

   Plasmid

   #80761

   Purpose

   Express Nuf2 N-terminally labeled with mTurquoise2

   Depositor

   Daniel Needleman

   Article

   Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

   Insert

   Nuf2 (NUF2 Human)
   

   Use

   Retroviral

   Tags

   mTurquoise2

   Expression

   Mammalian

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-puro-eGFP-HEC1-9A

   Plasmid

   #114023

   Purpose

   Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - 9A (NDC80) (NDC80 Human)
   

   Expression

   Mammalian

   Mutation

   Hec1 - 9A

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-Puro-FH-AGO2-5xA

   Plasmid

   #91992

   Purpose

   Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824A, S828A, T830A, S831A, S834A (5xA)

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-FNLSHiFi-P2A-Puro

    Plasmid

    #136902

    Purpose

    Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFi
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Mutation

    R691A

    Promoter

    EF1s

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. OCT4 GFP puro Donor 3

    Plasmid

    #22211

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    SA-OCT-GFP-2APuro-PA (POU5F1 Human)
    

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-HF1RA-P2A-Puro

    Plasmid

    #110853

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

    Plasmid

    #196710

    Purpose

    Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-VHL L188V-pBabe-puro

    Plasmid

    #19241

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Leucin 188 to Valin

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-puro-TTF-1-HDD

    Plasmid

    #74991

    Purpose

    Retroviral vector holding human TTF-1 (NKX2-1) with the homeodomain deleted

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    TTF-1 HDD mutant (NKX2-1 Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    HDD = homeodomain deleted

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe-Puro-RalA-V23

    Plasmid

    #15252

    Depositor

    William Hahn

    Article

    Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

    Insert

    v-ral simian leukemia viral oncogene homolog A (RALA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    changed 23rd amino acid to valine

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pGEMT-puroR-p2a-HA-halo

    Plasmid

    #161979

    Purpose

    homologous donor template for endogenous tagging

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    puroR-p2a-HA-halo-GSlinker
    

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. plko-puromycin-shTRX1-259

    Plasmid

    #110921

    Purpose

    To knockdown Thioredoxin-1

    Depositor

    Priyamvada Rai

    Article

    Young et al Biochem Biophys Res Commun. 2010 Feb 12;

    Insert

    TRX1 (TXN Human)
    

    Use

    Lentiviral

    Promoter

    unknown

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe-puro-Flag-TRAF5

    Plasmid

    #102679

    Purpose

    retroviral expression plasmid for TRAF5

    Depositor

    Hasam Habelhah

    Article

    Zhang et al J Mol Biol. 2009 Jun 12;389(3):495-510.

    Insert

    TRAF5 (Traf5 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid

    #69547

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge