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Str-KDEL_neomycin

Plasmid

#65306

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Tags

KDEL motif and Signal Sequence

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-300(HAT-)

Plasmid

#23254

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

p300 (EP300 Human)

Expression

Mammalian

Mutation

H1415A E1423A Y1424A L1428S Y1430A H1434A

Available Since

March 15, 2010

Availability

Academic Institutions and Nonprofits only

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Str-Ii_VSVG-SBP-mCherry

Plasmid

#65301

Purpose

synchronize trafficking of VSVG from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pKG3560_pHarbor-Rogi2_backbone

Plasmid

#239705

Purpose

PaqCI Golden Gate assembly plasmid encoding the backbone for Bxb1-mediated integration in the Rogi2 landing pad line

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN1-fDIO-tTA

Plasmid

#166597

Purpose

Encodes Flp-dependent tTA

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

tTA

Use

AAV

Promoter

human synapsin 1

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

Plasmid

#137993

Purpose

Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

Depositor

Amy Brock

Article

Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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VSVGwt-SBP-EGFP_puromycin

Plasmid

#65302

Purpose

VSVG reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shNC

Plasmid

#113535

Purpose

Control retroviral vector.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shNC (scrambled control shRNA)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Rap1b E63

Plasmid

#118328

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

Insert

Rap1b E63 (RAP1B Bovine)

Tags

GFP

Expression

Mammalian

Mutation

Q63E

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-Ii

Plasmid

#65313

Purpose

Cytoplasmic ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Use

Lentiviral

Mutation

Streptavidin in its cytoplasmic part

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pMito-PAGFP-FRB.

Plasmid

#60005

Purpose

Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Type

Empty backbone

Use

Knocksideways

Tags

FRB and PAGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ssSBP-EGFP

Plasmid

#65277

Purpose

to synchronize transport of secretory soluble GFP from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

EGFP

Tags

IL-2 signal sequence and Streptavidin Binding Pr…

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pJS167

Plasmid

#48881

Purpose

Positive feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

Inserts

araC gene with ssrA tag
monomeric yeast-enhanced green fluorescent protein

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Promoter

Para/lac

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN1-fDIO-rtTA

Plasmid

#166598

Purpose

Encodes Flp-dependent rtTA

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

rtTA

Use

AAV

Promoter

human synapsin 1

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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GST-Pin1

Plasmid

#19027

Depositor

Michael Yaffe

Article

Yaffe et al Science. 1997 Dec 12. 278(5345):1957-60.

Insert

Pin1 (PIN1 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE

Plasmid

#137994

Purpose

Backbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcoding

Depositor

Amy Brock

Article

Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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p1242 3x-KB-L

Plasmid

#26699

Depositor

Bill Sugden

Article

Mitchell et al J Virol. 1995 May . 69(5):2968-76.

Insert

3x MHC I KB element - luciferase (NFKB1 Human, Photinus pyralis)

Use

Luciferase

Expression

Mammalian

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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pSPORT-RREB1

Plasmid

#41145

Depositor

Joshua Mendell

Article

Kent et al Oncogene. 2012 Jul 2. doi: 10.1038/onc.2

Insert

RREB-1 (RREB1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

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Ii-Str_SBP-EGFP-Ecadherin

Plasmid

#65288

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

Plasmid

#84250

Purpose

Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgTRE3G

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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