We narrowed to 74,937 results for: Rest

1. pET28-OmpT

   Plasmid

   #68862

   Purpose

   construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

   Depositor

   Neil Kelleher

   Article

   Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

   Insert

   OmpT (ompT )
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOVC1_X_ScPH1-S

   Plasmid

   #131193

   Purpose

   Expresses ScPH1-S (Synechosystis PCC6803) preceded by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   ScPH1-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQF-mNGsacB

   Plasmid

   #203673

   Purpose

   pQF with mNeonGreen and sacB under control of PQ5

   Depositor

   Urs Jenal

   Article

   Klotz et al Appl Environ Microbiol. 2023 May 18:e002

   Inserts

   mNeonGreen
   sacB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Has synonymous (silent) mutations to remove BsrGI…

   Promoter

   PQ5

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFUS_B10

   Plasmid

   #31027

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   LacZ + BsaI restriction sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOVC1_AtPIF6_X

   Plasmid

   #131182

   Purpose

   Expresses AtPIF6 (Arabidopsis thaliana) followed by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   AtPIF6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-optoB2-SS-YFP

   Plasmid

   #75270

   Purpose

   Arrestin independent version of optoB2 with serine mutations at S362A,S363G

   Depositor

   Michael Bruchas

   Article

   Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

   Insert

   Arrestin independent version of optoB2
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   serine mutations at S362A,S363G

   Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJ211_WT_hi

   Plasmid

   #128850

   Purpose

   High copy number plasmid for expression of E. coli flagellin from its natural promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS_KS_attB2_SA(0)_T2AGAL4

   Plasmid

   #125211

   Purpose

   Artificial phase 0 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

   Depositor

   Herman Dierick

   Article

   Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

   Insert

   GAL4
   

   Use

   Other

   Tags

   T2A

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBS_KS_attB2_SA(2)_T2AGAL4

   Plasmid

   #125213

   Purpose

   Artificial phase 2 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

   Depositor

   Herman Dierick

   Article

   Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

   Insert

   GAL4
   

   Use

   Other

   Tags

   T2A

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS_KS_attB2_SA(1)_T2AGAL4

    Plasmid

    #125212

    Purpose

    Artificial phase 1 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL4
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pREDusk-StrR-MCS

    Plasmid

    #188972

    Purpose

    For red light-repressed bacterial expression of gene of interest (StrR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFUS_A

    Plasmid

    #31028

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pME nls-eos

    Plasmid

    #73791

    Purpose

    Middle entry clone with photoconvertible eos conjugated to a nuclear localization signal

    Depositor

    David Raible

    Article

    Curran et al Dev Biol. 2010 Aug 1;344(1):107-18. doi:

    Insert

    nls-eos
    

    Use

    Middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. JGE301_pAAV-hSyn-DIO-Flag-hM3Dq-V2(2D)-ABIcs-mCherry

    Plasmid

    #107246

    Purpose

    hM3DREADD fused to ABIcs for abscisic acid mediated recruitment of PYLcs labelled proteins.

    Depositor

    Bryan Roth

    Article

    Roth et al bioRxiv 251769

    Insert

    hM3DREADD
    

    Use

    AAV

    Tags

    ABIcs, FLAG, and mCherry

    Expression

    Mutation

    T to D and S to D mutations in V2-tail

    Promoter

    hSYN

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMVTCRa_candidate5

    Plasmid

    #164999

    Purpose

    Expression of candidate TCR #5 TCRalpha cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    TRAV (TRA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMVTCRb_candidate5

    Plasmid

    #165000

    Purpose

    Expression of candidate TCR #5 TCRbeta cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    TRB (TRB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJOG022

    Plasmid

    #105384

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    promotor Zea mays Ubiquitin (1500bp)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI/ BpiI restriction sites removed

    Promoter

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CMVTCRb_candidate7

    Plasmid

    #165004

    Purpose

    Expression of candidate TCR #7 TCRbeta cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    TRB (TRB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMVTCRa_candidate7

    Plasmid

    #165003

    Purpose

    Expression of candidate TCR #7 TCRalpha cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    TRAV (TRA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJ291_WT_med

    Plasmid

    #128856

    Purpose

    Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge