We narrowed to 24,871 results for: Spr

1. pBBK23 pCAS-Tyr-[gRNA: 6=ARS416] (SplitHygR, AmpR)

   Plasmid

   #179007

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, AmpR)

   Plasmid

   #179006

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBBK17 pCAS-Tyr-[gRNA: 7=HIS3] (SplitKanR, AmpR)

   Plasmid

   #179001

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBBK16 pCAS-Tyr-[gRNA: 6=ARS416] (SplitKanR, AmpR)

   Plasmid

   #179000

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBBK15 pCAS-Tyr-[gRNA: 5=ARS308] (SplitKanR, AmpR)

   Plasmid

   #178999

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBBK14 pCAS-Tyr-[gRNA: 4=XII-5] (SplitKanR, AmpR)

   Plasmid

   #178998

   Purpose

   Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBBK13 pCAS-Tyr-[gRNA: 3=XII-2] (SplitKanR, AmpR)

   Plasmid

   #178997

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 1137B=Hsp70Bb-Cas9

   Plasmid

   #153284

   Purpose

   The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Insert

   pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Promoter

   Dmel Hsp70Bb

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS424-ysg(G:H)

   Plasmid

   #138257

   Purpose

   Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   GFP targeting sgRNAs G and H
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEG302 22aa SunTag VP64 gRNA17 (FWA)

    Plasmid

    #120250

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459V2.0+N497A+Q695A+Q926A

    Plasmid

    #108300

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N497A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSC45

    Plasmid

    #104819

    Purpose

    CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-2). Also expresses Cas9 from Gmubi promoter.

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.04g057400
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSC29

    Plasmid

    #104803

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr4g094545 (Hen1). Also expresses Cas9 from Gmubi promoter.

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Medtr4g094545
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. px330-MRPsg3

    Plasmid

    #87191

    Purpose

    disruption of human RMRP gene via CRISPR-Cas9, guide 3

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    human MRP RNA CRISPR guide #3 (RMRP Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKSE401

    Plasmid

    #62202

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9(BB)-2A-Puro (PX459) V2.0

    Plasmid

    #62988

    Purpose

    Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9-2A-Puro V2.0
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ173

    Plasmid

    #99907

    Purpose

    Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Insert

    pco-dCas9-VP64-T2A-MS2-VP64
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-FLEX-SaCas9-U6-sgRNA

    Plasmid

    #124844

    Purpose

    Vector for Cre-dependent expression of SaCas9

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SaCas9, gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS and NLS-3xHA

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHSE401

    Plasmid

    #62201

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiSAMv2

    Plasmid

    #75112

    Purpose

    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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