165,198 results

1. pCMV6-AC EJ7-GFP

   Plasmid

   #113617

   Purpose

   Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   EJ7-GFP variant of EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. NNMT

   Plasmid

   #40734

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NNMT (NNMT Human)
   

   Use

   Tags

   His Thrombin

   Expression

   Bacterial

   Mutation

   K100A:E101A:E103A

   Promoter

   Available since

   Oct. 18, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pAd5-B6 ∆E3-GFP

   Plasmid

   #179201

   Purpose

   Block 6 with deletion in E3 and insertion of GFP expression cassette

   Depositor

   Fred Bunz

   Article

   Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

   Insert

   Modified block 6, with deletion in E3 and insertion of GFP expression cassette
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-DIO ChRmine-eYFP-WPRE

   Plasmid

   #130996

   Purpose

   Double floxed ChRmine-eYFP under the control of Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   eYFP

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. SETD2-GFP

   Plasmid

   #80653

   Purpose

   mammalian expression of SETD2-GFP

   Depositor

   Sérgio De Almeida

   Article

   Carvalho et al Elife. 2014 May 6;3:e02482. doi: 10.7554

   Insert

   SETD2 (SETD2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. p201H Cas9

   Plasmid

   #59176

   Purpose

   Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   hph
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S and StUbi-3P

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYC-N

   Plasmid

   #207113

   Purpose

   HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

   Depositor

   Yifan Cheng

   Article

   Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

   Plasmid

   #37120

   Purpose

   DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   TdTomato-EGFP
   

   Use

   AAV and Cre/Lox; Cre-switch

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

   Plasmid

   #185918

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-Ykt6

    Plasmid

    #209888

    Purpose

    Expresses mCherry tagged Ykt6 in mammalian cells

    Depositor

    Geert van den Bogaart

    Insert

    Ykt6
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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