161,511 results

1. EF1a-dCasRx-RBM25

   Plasmid

   #221001

   Purpose

   Transient expression of dCasRx-RBM25. EF1a promoter.

   Depositor

   Mikko Taipale, Benjamin Blencowe

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   dCasRx-RBM25
   

   Use

   CRISPR

   Tags

   NLS and NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-HA-CUL1 Y42A/M43A

   Plasmid

   #19940

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

   Insert

   cullin 1 Y42A/M43A (CUL1 Human)
   

   Use

   Tags

   HA2

   Expression

   Mammalian

   Mutation

   CUL1 with a SKP1 binding mutant

   Promoter

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pLX-sgRNA

   Plasmid

   #50662

   Purpose

   Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

   Depositor

   David Sabatini, Eric Lander

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   sgAAVS1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCLX-PGK-hTERT

   Plasmid

   #114315

   Purpose

   Constitutive expression of the hTert protein

   Depositor

   Patrick Salmon

   Insert

   hTERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMIR-DsRed-IRES-His-Halo-Tev-Keap1

   Plasmid

   #58240

   Purpose

   expresses halo tagged human Keap1 protein

   Depositor

   Yimon Aye

   Article

   Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

   Insert

   Kelch like ECH associated protein 1 (KEAP1 Human)
   

   Use

   Tags

   His-Halo-Tev

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. Ube1/PET21d

   Plasmid

   #34965

   Depositor

   Cynthia Wolberger

   Article

   Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

   Insert

   Ube1 (UBA1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-wGBD

   Plasmid

   #26734

   Depositor

   William Bement

   Article

   Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

   Insert

   Neuronal Wiskott-Aldrich Syndrome protein (WASL Human)
   

   Use

   Reporter for cdc42

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Cdc42 binding domain of N-WASP

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC18T-mini-Tn7T-Gm-Pc-mNeonGreen

   Plasmid

   #205022

   Purpose

   mini-Tn7 vector for insertion of mNeonGreen (codon optimized for P. fluorescens) at bacterial attTn7 site

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(+)/Luc2=tdT

   Plasmid

   #32904

   Purpose

   Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.

   Depositor

   Christopher Contag

   Article

   Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.

   Inserts

   Firefly Luciferase
   tandem Tomato Red Fluorescent Protein
   

   Use

   Tags

   tdTomato red fluorescent protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE and n/a

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. phOct4-EGFP

    Plasmid

    #38776

    Depositor

    Wei Cui

    Article

    Gerrard et al Stem Cells. 2005;23(1):124-33.

    Insert

    Oct-4 promoter (POU5F1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    human OCT4

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge