We narrowed to 4,319 results for: 1-Oct

1. pCS2+ 3xFLAG-FEM1B WT

   Plasmid

   #230993

   Purpose

   Transient overexpression of mouse FEM1B in mammalian cells

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Insert

   FEM1B (Fem1b Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

   Plasmid

   #173208

   Purpose

   HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1-T804N mScarlet-I-TOSI donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK1

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cav2.2 BBS exon37a pCAGGS

   Plasmid

   #206086

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and alternative exon 37a

   Depositor

   Annette Dolphin

   Article

   Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/B-actin

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP Cav2.2 HA pCAGGS

   Plasmid

   #206054

   Purpose

   expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

   Depositor

   Annette Dolphin

   Article

   Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/B-actin

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP Cav2.2 HA pcDNA3

   Plasmid

   #206053

   Purpose

   expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

   Depositor

   Annette Dolphin

   Article

   Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBAD-HisD-BR1

   Plasmid

   #192816

   Purpose

   Expresses BR1 in bacteria

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   BR1 (BRI1 Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Ppst93-MCRaeo-NG mcrBGA

   Plasmid

   #192769

   Purpose

   Expresses NG-tagged MCRaeoBGA in Methanococcus maripaludis

   Depositor

   William B. Whitman

   Article

   Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

   Insert

   Methyl coenzyme M reductase
   

   Use

   Synthetic Biology; Heterologous expression in met…

   Tags

   Flag-Strep2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. Ppst93-MCRaeo-NG mcrBCGA

   Plasmid

   #192768

   Purpose

   Expresses NG-tagged MCRaeoBCGA in Methanococcus maripaludis

   Depositor

   William B. Whitman

   Article

   Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

   Insert

   Methyl coenzyme M reductase
   

   Use

   Synthetic Biology; Heterologous expression in met…

   Tags

   Flag-Strep2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. Ppst93-MCRaeo-NG mcrBDGA

   Plasmid

   #192767

   Purpose

   Expresses NG-tagged MCRaeoBDGA in Methanococcus maripaludis

   Depositor

   William B. Whitman

   Article

   Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

   Insert

   Methyl coenzyme M reductase
   

   Use

   Synthetic Biology; Heterologous expression in met…

   Tags

   Flag-Strep2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pYI006

    Plasmid

    #170840

    Purpose

    FRO6p::GUSPlus::tOCS in pFP100

    Depositor

    Christian Fankhauser

    Article

    Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

    Insert

    FRO6p::GUSPlus::tOCS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    FRO6

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDIV495

    Plasmid

    #177702

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ATP1A1_G3_EBFP_Nick_Dual_sgRNA

    Plasmid

    #178094

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + EBFP nick sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ATP1A1_G3_EGFP_Nick_Dual_sgRNA

    Plasmid

    #178095

    Purpose

    Control vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + EGFP nick sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ATP1A1_G3_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid

    #178096

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + MTOR nick exon-44 sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA

    Plasmid

    #178099

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + MTOR nick intron-45 sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ATP1A1_G4_Q118R_MTOR_F2108L_Dual_pegRNA

    Plasmid

    #178102

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-F2108L pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + MTOR-F2108L pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ATP1A1_G4_Q118R_MTOR_I2017T_Dual_pegRNA

    Plasmid

    #178103

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-I2017T pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + MTOR-I2017T pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ATP1A1_G8_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid

    #178105

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G8 + MTOR nick exon-44 sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ATP1A1_G8_MTOR_Nick_Intron-45_Dual_sgRNA

    Plasmid

    #178106

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G8 + MTOR nick intron-45 sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ATP1A1_G6_T804N_MTOR_I2017T_Dual_pegRNA

    Plasmid

    #178108

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-I2017T pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G6 T804N pegRNA + MTOR-I2017T pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge