We narrowed to 3,144 results for: PPL;

1. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

   Plasmid

   #233080

   Purpose

   Expression vector for sgRNA-S1b and eCas12f1i

   Depositor

   Kyoungmi Kim

   Article

   Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

   Insert

   U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, CBh

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3/mStayGold(c4)=UtrCH

   Plasmid

   #212020

   Purpose

   For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   UtrCH
   

   Use

   Tags

   mStayGold(c4)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a

   Plasmid

   #233079

   Purpose

   Expression vector for sgRNA-S1b and eCas12f1a

   Depositor

   Kyoungmi Kim

   Article

   Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

   Insert

   U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, CBh

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG PSAM4 5HT3 LC IRES GFP

   Plasmid

   #123066

   Purpose

   Chemogenetic activator expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L131G Q139L Y217F

   Promoter

   CAG

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T-1 3xFlag-STAT1

   Plasmid

   #82722

   Purpose

   Expresses human STAT1

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   STAT1 (STAT1 Human)
   

   Use

   Tags

   3xFLAG and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1

   Plasmid

   #233078

   Purpose

   Expression vector for sgRNA-S1b and eCas12f1

   Depositor

   Kyoungmi Kim

   Article

   Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

   Insert

   U6-sgRNA-S1b (BsaI)-CBh-bpNLS-eCas12f1-2xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, CBh

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEasyG2_mic

   Plasmid

   #184916

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMC234r-ccdB/TPK2

   Plasmid

   #176179

   Purpose

   yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   dropout cassette containing toxic genes ccdB and TPK2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUltra-sY

   Plasmid

   #82417

   Purpose

   Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons

   Depositor

   Chang Liu

   Article

   Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

   Insert

   Methanococcus jannaschii aaRS for sulfotyrosine
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…

   Promoter

   tacI

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5E-mmCdh5pro (JDW 1239)

    Plasmid

    #224478

    Purpose

    Gateway compatible 5' entry clone containing the Murine Cdh5 promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Cdh5 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-RO1.7-RPE65

    Plasmid

    #206346

    Purpose

    AAV plasmid expressing RPE65 in photoreceptors

    Depositor

    Connie Cepko

    Article

    Xue et al Proc Natl Acad Sci U S A. 2023 Jun 6;120

    Insert

    Rpe65 (Rpe65 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    450-Met (C57BL6 natural variation)

    Promoter

    1.7kb human red opsin

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX303-ABI1-dCas9

    Plasmid

    #154474

    Purpose

    Expresses ABI1 fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ABI1 (ABI1 Mustard Weed)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Includes ABI1 aa 126-423, D143A

    Promoter

    CMV

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-4T-1 3xFlag-MAPKAPK2

    Plasmid

    #82720

    Purpose

    Expresses human MAPKAPK2

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MAPKAPK2 (MAPKAPK2 Human)
    

    Use

    Tags

    3xFLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

14. p5E-hs_ICAM_short (JDW 1046)

    Plasmid

    #224480

    Purpose

    Gateway compatible 5' entry clone containing the Human minimal ICAM2 promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    min-ICAM2 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC-fFuCas9-HTBNLS-hph

    Plasmid

    #121092

    Purpose

    Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

    Depositor

    Xiao-Jun Ji

    Article

    Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

    Insert

    Cas9 and hygromycin resist gene
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    gpdA;trpC

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

    Plasmid

    #110560

    Purpose

    miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    stRBS-lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaSR-PrhaBAD

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

    Plasmid

    #167901

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p5E-CAGGS (JDW 912)

    Plasmid

    #224472

    Purpose

    Gateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    CAGGS Promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. p5E-EF1a (JDW 1164)

    Plasmid

    #224473

    Purpose

    Gateway compatible 5' entry clone containing the human EF1a promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EF1a promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV2_CAG_oROS-G

    Plasmid

    #216115

    Purpose

    Encodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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