We narrowed to 3,906 results for: SPL

1. pMSCV-IRES-GFP-3xFLAG-MyD88-V5

   Plasmid

   #131351

   Purpose

   Expresses MyD88 tagged with a 3xFLAG tag at its N-terminus and a V5 tag at its C-terminus

   Depositor

   Jonathan Kagan

   Article

   Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

   Insert

   MyD88 (Myd88 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSiMPla_N + pSiMPla_C

   Plasmid

   #134314

   Purpose

   Maintenance of 2 plasmids with a single antibiotic in E. coli

   Depositor

   Barbara Di Ventura

   Article

   Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

   Insert

   ampR_gp41-1 N-intein + ampR_gp41-1 C-intein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Expression scaffold for dCas9+sgRNA

   Plasmid

   #171799

   Purpose

   PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

   Depositor

   Stephen Elledge

   Article

   Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

   Insert

   PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV/MASC_(GLuc)_TOP1

   Plasmid

   #68439

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. PEP112E

   Plasmid

   #97398

   Purpose

   binary construct of mitochondria-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   Mitochondria target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. NIR-AG

   Plasmid

   #210728

   Purpose

   NIR light-activated GSDMD

   Depositor

   Vladislav Verkhusha

   Article

   Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

   Insert

   NIR-AG (GSDMD Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE80l A-CC-A

   Plasmid

   #196478

   Purpose

   Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

   Depositor

   Fei Sun

   Article

   Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

   Insert

   A-CC-A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PEP111E

   Plasmid

   #97397

   Purpose

   binary construct of plastid-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   Plastid target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT-TetO-U1-70K-fSNAPc

   Plasmid

   #106987

   Purpose

   open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   U1-70K (SNRNP70 Human)
   

   Use

   Tags

   fSNAP

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)

    Plasmid

    #140226

    Purpose

    Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistance

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    Luciferase + crIFNg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 FLIPE-100u

    Plasmid

    #13543

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    S118W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE

    Plasmid

    #140229

    Purpose

    Dox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFP

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    AntiCRISPR protein AcrVA1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUBC_HA_2xPP7-VP64

    Plasmid

    #68418

    Purpose

    Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    PP7(deltaFG)
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Mutation

    PP7: [delta]67_74 (non-aggregating)

    Promoter

    human UbC

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJYDN3p

    Plasmid

    #162457

    Purpose

    Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.

    Depositor

    Gideon Schreiber

    Article

    Zahradník et al bioRxiv Preprint 2020

    Insert

    Aga2p-eUnaG2 (AGA2 )
    

    Use

    Shuttle vector bacteria/yeast

    Tags

    ALFA tag, HA and myc tags, and eUnaG2 fluorescent…

    Expression

    Mutation

    Promoter

    GAL1

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-BirA-eCPX-AP

    Plasmid

    #121907

    Purpose

    Expresses BirA and eCPX-AP

    Depositor

    Per Svenningsen

    Article

    Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

    Inserts

    BirA (birA Synthetic)
    Enhanced Circularly Permuted Outer Membrane Protein X
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and pBAD promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET:GACT:GFP

    Plasmid

    #60734

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU1/Sm/3' Box_(GLuc)_TOP1

    Plasmid

    #68437

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U1

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. PEP108E

    Plasmid

    #97394

    Purpose

    binary construct of plasma membrane-targeted sfGFP1-10 for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    sfGFP1-10OPT
    

    Use

    Tags

    Plasma membrane target

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSC-SmBiT

    Plasmid

    #207632

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-SmBiT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3-ySet2

    Plasmid

    #51698

    Purpose

    Expresses full length yeast Set 2 gene in a mamalian expression vector

    Depositor

    Tom Misteli

    Article

    Luco et al Science. 2010 Feb 19;327(5968):996-1000.

    Insert

    yeast Set2 (SET2 Budding Yeast)
    

    Use

    Tags

    C-terminal FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only