We narrowed to 7,267 results for: trac

1. pTSK573

   Plasmid

   #190881

   Purpose

   For C-terminal -Halo Tagging of a gene of interest with TRP1 selection marker in yeast S. cerevisiae

   Depositor

   Gunjan Mehta, Tatiana Karpova

   Article

   Podh et al STAR Protoc. 2022 Dec 16;3(4):101900. do

   Insert

   HaloTag
   

   Use

   Bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-fDIO-tdTomato-WPRE

   Plasmid

   #187112

   Purpose

   FLP-dependent expression of tdTomato under EF1a promoter

   Depositor

   Yongling Zhu

   Article

   Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2370-Tier1-PhCMV-TetR_F-type

   Plasmid

   #169573

   Purpose

   Tier-1 vector encoding PhCMV-driven tetracycline-dependent F-type-based transactiavtor (PhCMV-TetR-F-type-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled transactivator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEM262 - pDESTR4-R3 mosTI hygroR target

   Plasmid

   #159840

   Purpose

   Single copy or array insertion by MosTI using split hygroR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   pDESTR4-R3 mosTI hygroR target
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMC-9-35S-11

   Plasmid

   #176881

   Purpose

   MoClo Level 0 plasmid, contains 35S

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   35S terminator
   

   Use

   Tags

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. gSCPL35-mRFP

   Plasmid

   #181960

   Purpose

   SCPL35 protein tagged with mRFP, under control of native promoter

   Depositor

   George Haughn

   Article

   Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

   Insert

   SCPL35
   

   Use

   Tags

   mRFP

   Expression

   Plant

   Mutation

   Promoter

   native SCPL35

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-tdMCP2-GyG-(GGS)X6-HaloTag C261

   Plasmid

   #171434

   Purpose

   Expression of tdMCP2-GyG-(GGS)X6-HaloTag C261 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   tdMCP2-GyG-(GGS)X6-HaloTag C261
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCFJ2365 - Intron GG1 - 250bp no PATC

   Plasmid

   #159883

   Purpose

   Golden Gate compatible intron with no PATCs for negative controls

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG1 - 250bp no PATC
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFJ2345 - Intron GG2 - 250bp no PATC

   Plasmid

   #159884

   Purpose

   Golden Gate compatible intron with no PATCs for negative controls

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG2 - 250bp no PATC
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCFJ2366 - intron GG3 - 250bp no PATC

    Plasmid

    #159885

    Purpose

    Golden Gate compatible intron with no PATCs for negative controls

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG3 - 250bp no PATC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSEM271 - MCS mosTI NeoR target

    Plasmid

    #159830

    Purpose

    Single copy or array insertion by MosTI using split NeoR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    MCS mosTI NeoR target
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSEM254 - sgRNA mosTI neoR

    Plasmid

    #159831

    Purpose

    Single copy or array insertion by MosTI using split NeoR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI neoR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSEM322 - array co-injection fragment mosTI neoR

    Plasmid

    #159832

    Purpose

    Single copy or array insertion by MosTI using split NeoR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI neoR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV RhoB YFP MS2

    Plasmid

    #131777

    Purpose

    RhoB upstream msfYFP 24xMS2 RhoB downstream for CRISPR tagging

    Depositor

    Jared Toettcher

    Article

    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

    Insert

    msfYFP - 24x MS2 loops
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV Btg2 YFP MS2

    Plasmid

    #131776

    Purpose

    Btg2 upstream msfYFP 24xMS2 Btg2 downstream for CRISPR tagging

    Depositor

    Jared Toettcher

    Article

    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

    Insert

    msfYFP - 24x MS2 loops
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MRLC-DD-pIRES-EGFP-AP4-mito

    Plasmid

    #101287

    Purpose

    To define the role of Mena/VASP in tension-sensitive actin assembly

    Depositor

    Alpha Yap

    Article

    Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

    Insert

    MRLC-DD and AP4-Mito
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV.UBI.GFP.miR292T

    Plasmid

    #115972

    Purpose

    miR-292 regulated GFP expression

    Depositor

    Johan Jakobsson

    Article

    Sachdeva et al Proc Natl Acad Sci U S A. 2010 Jun 22;10

    Insert

    GFP.miR292T
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    ubiquitin

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-kanMX6-P81nmt1-mEGFP

    Plasmid

    #105143

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

    Insert

    kanMX6-P81nmt1-mEGFP
    

    Use

    Tags

    81nmt1-mEGFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    81nmt1

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a - MPX

    Plasmid

    #89619

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Mitocondrial Tryparedoxin peroxidase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21a - EtfA

    Plasmid

    #89616

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Electron transport flavoprotein alpha subunit
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge