165,586 results

1. pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

   Plasmid

   #175544

   Purpose

   Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   IgKchL_HA-HaloTag9-myc_PDGFRtmb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-C-STAT1-mGFP-P2A-Puro

   Plasmid

   #199345

   Purpose

   express murine STAT1 protein fused with mGFP

   Depositor

   Massimo Mallardo

   Article

   Caiazza et al Int J Mol Sci. 2022 Nov 17;23(22):14232.

   Insert

   signal transducer and activator of transcription 1 (Stat1 Mouse)
   

   Use

   Lentiviral

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7_CC_PEmax_IVT_Template

   Plasmid

   #216793

   Purpose

   Template for in vitro transcription of PEmax.

   Depositor

   Daniel Bauer

   Article

   Levesque et al Nat Biotechnol. 2024 May 28. doi: 10.103

   Insert

   PEmax
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-SIRT1 H363Y

   Plasmid

   #1792

   Depositor

   Michael Greenberg

   Article

   Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

   Insert

   SIRT1 (SIRT1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H363Y, deacetylase domain mutation

   Promoter

   Available since

   May 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK172-6xHis-0N4R-P301S-tau-HiBiT

   Plasmid

   #187835

   Purpose

   Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

   Depositor

   William McEwan

   Article

   Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

   Insert

   6XHis-0N4R-P301S-tau-HiBiT
   

   Use

   Tags

   6xHistidine and HiBiT

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG_IGF1R_EGFP

   Plasmid

   #199628

   Purpose

   FRET donor for IGF1R sensor

   Depositor

   Ryohei Yasuda

   Article

   Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

   Insert

   IGF1R (IGF1R Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-BirA*-dCas9-eGFP

   Plasmid

   #84779

   Purpose

   Expresses BirA*-dCas9-eGFP in mammalian cells.

   Depositor

   Heinrich Leonhardt

   Article

   Schmidtmann et al Nucleus. 2016 Sep 27:0.

   Insert

   BirA*-dCas9-eGFP
   

   Use

   CRISPR

   Tags

   cMyc

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-N E4orf1

   Plasmid

   #38063

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   E4 orf1
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   PKG

   Available since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDe-CAS9

   Plasmid

   #61433

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PcUBI4-2

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-mCherry (AAV8)

    Viral Prep

    #114470-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-mCherry (#114470). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry plasmid DNA. EF1a-driven mCherry expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    mCherry

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only