We narrowed to 34,868 results for: CaS;

1. pMS30: pTarget(PmcCAST)

   Plasmid

   #168163

   Purpose

   Target plasmid containing PmcPSP1 and attachment site for PmcCAST.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PAM for PmcCAST + PmcPSP1
   tRNA-Val
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3xFLAG-dCas9/pMSCVneo

   Plasmid

   #134982

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xFLAG-dCas9/pMSCVpuro

   Plasmid

   #134983

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMB1-A-ptet-dCas9

   Plasmid

   #190129

   Purpose

   Level 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRi

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMB1-A-pBAD-dCas9

   Plasmid

   #190132

   Purpose

   Level 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRi

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRS-LacI-dCas9

   Plasmid

   #220194

   Purpose

   mini-Tn7 dCas9 expression vector - LacI/pLac promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   LacI/pLac

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-nSpCas9-TadA8e

   Plasmid

   #209044

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TadA8e-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. zCREST3:Cas9green; EGF_gRNA

   Plasmid

   #199333

   Purpose

   pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   zCREST3
   Cas9
   nrg1 EGF sgRNA
   

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AsCas12a-2C-NLS in pCSDest

   Plasmid

   #126637

   Purpose

   Expresses AsCas12a-2C-NLS in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   AsCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94 promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. ASC-CASP1 Octamer

    Plasmid

    #164032

    Purpose

    Bacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamer

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CASP1, ASC (CASP1, PYCARD Human)
    

    Use

    Tags

    His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    W169G (ASC), G20K (CASP1)

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. RCAS(A) mdnTcf4Ben (CT#1130)

    Plasmid

    #14020

    Depositor

    Cliff Tabin

    Article

    Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

    Insert

    Tcf4B (Tcf7l2 Mouse)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Dominant negative Tcf4B, engrailed fusion

    Promoter

    Available since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pSpCas9(BB)-2A-GFP-G3BP1(1)

    Plasmid

    #136058

    Purpose

    G3BP1 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (GTAGTCCCCTGCTGGTCGGGC)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pShoCAST-sgRNA_entry (BO1)

    Plasmid

    #181786

    Purpose

    Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac and J23119

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xFLAG-dCas9/pMXs-IG

    Plasmid

    #51258

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-TO-Sp dCas9-mVenusN

    Plasmid

    #191396

    Purpose

    SpdCas9 fused to N-terminal part of mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    N-terminal part of split mVenus
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC1804_pmax-dCasRx-RBM38

    Plasmid

    #118638

    Purpose

    Transient transfection; Expresses dCasRx-RBM38; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    dCasRx-RBM38
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDicAID_nCas9-PmCDA-2A-NptII_ETR

    Plasmid

    #91695

    Purpose

    Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A for nickase Cas9

    Promoter

    PcUbi

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. plenti-As-Cas12a-2xNLS

    Plasmid

    #155047

    Purpose

    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    AsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS, Myc-tag and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC149-pCR8-dCas9VP160

    Plasmid

    #48221

    Purpose

    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP160

    Expression

    Mutation

    D10A;H840A nuclease-deficient

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFREE2-SpCas9-sgRNA

    Plasmid

    #179582

    Purpose

    Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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