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PB-TetON-dual-SunTag-Hygro

Plasmid

#121119

Purpose

Endogenous gene activation with the SunTag CRISPR activation system

Depositor

Pablo Navarro

Article

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Insert

dCas9-GCN4x10_scFv-VP64

Use

CRISPR

Expression

Mammalian

Promoter

Tet-ON

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-siRNA control

Plasmid

#10900

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siGFP

Expression

Mammalian

Available Since

Feb. 17, 2006

Availability

Academic Institutions and Nonprofits only
pAd-Track SIRT1

Plasmid

#8440

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Expression

Mammalian

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Mito-Pericam

Plasmid

#87381

Purpose

Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.

Depositor

Björn Stork

Article

Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

Insert

Mito-Pericam [ratiometric]

Use

Retroviral

Expression

Mammalian

Promoter

Retroviral 5' LTR promoter

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 NSP3_nostop

Plasmid

#149306

Purpose

Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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SBE2-Luc

Plasmid

#16500

Depositor

Bert Vogelstein

Article

Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

Insert

Smad binding element

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81

Plasmid

#100157

Purpose

Expresses MUS81

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

MUS81 (MUS81 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pEF-HA-NBP1 (P#1261)

Plasmid

#14586

Depositor

Matija Peterlin

Article

Lu et al Immunity. 1998 May . 8(5):647-56.

Insert

ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pEB1-mGFPmut2

Plasmid

#103980

Purpose

Plasmid encoding mGFPmut2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65A, V68L, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-NLGN3 WT

Plasmid

#59318

Purpose

Expression plasmid of HA-tagged wildtype human Neuroligin 3

Depositor

Peter Scheiffele

Article

Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

Insert

NLGN3 (NLGN3 Human)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-Smad4-Flag

Plasmid

#37041

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2011 Feb 1;71(3):998-1008. E

Insert

Smad4 (SMAD4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

5'LTR

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag mTOR S2035T

Plasmid

#26604

Depositor

Jie Chen

Article

Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

Insert

mTOR (MTOR Human)

Tags

Flag

Expression

Mammalian

Mutation

S2035T

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
pYpet-C1

Plasmid

#22780

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Ypet

Expression

Mammalian

Mutation

Control for intermolecular Rac1 biosensor FRET. S…

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only
MSCV FLIP FF

Plasmid

#19744

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting Firefly luciferase

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pSUPER.PKCzeta.RNAi

Plasmid

#10803

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKC zeta RNAi (PRKCZ Human)

Use

RNAi

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pT3-EFa1-HA-Jag1

Plasmid

#46051

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Tags

HA tag

Expression

Mammalian

Promoter

EF1a

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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FLAG-NLRP10

Plasmid

#38141

Depositor

Thomas Kufer

Article

Lautz et al Cell Microbiol. 2012 Jun 4. doi: 10.1111

Insert

NLRP10 (NLRP10 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro1 T18N

Plasmid

#47890

Purpose

Expresses myc tagged Miro1 T18N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 T18N (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

T18N, dominant negative mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP_PCNA_IRES_puro2b

Plasmid

#21048

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

hPCNA

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only

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pYFP-NGAT

Plasmid

#11383

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)

Tags

YFP

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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