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Plasmid#214837PurposeThis plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-GFPnanobody
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 GFPNanobody_SNAP
Plasmid#82711PurposeExpresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.DepositorInsertGFP Nanobody
UseTags6xHis Purification tag, FLAG purification tag, an…ExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-GFP-Nanobody
Plasmid#61838PurposeExpresses GST-tagged anti-GFP Nanobody in E. coli cells.DepositorInsertGFP-Nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINE GFP nanobody
Plasmid#49172PurposeBacterial expression of GFP nano bodyDepositorInsertGFP nanobody
UseTagsHHHHHHExpressionBacterial and MammalianMutationPromoterT7Available sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
UseTagsHalo and His6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b_1_anti-GFP-nanobody
Plasmid#210394PurposeExpress anti-GFP nanobody in E. coliDepositorInsert1_anti-GFP-nanobody
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3.0_nucNanobody IRES EGFP
Plasmid#136619PurposePlasmid encoding nuclear localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertSNAP-Tag-EnhancerNb-NLS (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoNanobody IRES EGFP
Plasmid#136621PurposePlasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertNTOM20-SNAP-Tag-EnhancerNb (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynNanobody IRES EGFP
Plasmid#136624PurposePlasmid encoding outer inner membrane localization of the Enhancer nanobody GFP as a SNAP-Tag-fusion and EGFPDepositorInsertLyn11-SNAP-Tag-EnhancerNb (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-anti-AlfaTag nanobody
Plasmid#171818PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertcfSGFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
nanobody(AntiGFP)-HaloTag-iLID
Plasmid#191453PurposeLocalizing iLID with GFP-tagged proteinsDepositorInsertnanobody(AntiGFP)-HaloTag-iLID
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
IC65 pEGFPN1 anti-IRSp53 nanobody
Plasmid#135227PurposeMammalian expression of anti-IRSp53 (Human) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-IRSp53 (Human) recombinant llama nanobody IC65 (BAIAP2 L. glama (llama))
UseTags6xHis-HA-EGFPExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
AC50 pEGFPN1 anti-AMIGO-1 nanobody
Plasmid#135225PurposeMammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-AMIGO-1 (Mouse) recombinant llama nanobody AC50 (Amigo1 L. glama (llama))
UseTags6xHis-HA-EGFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
GC52 pEGFPN1 anti-Gephyrin nanobody
Plasmid#135224PurposeMammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))
UseTags6xHis-HA-EGFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMD1 anti-GFP nanobody-Tn5
Plasmid#215567PurposeExpresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tagDepositorInsertanti-GFP nanobody-Tn5 fusion
UseTags3xFlag and intein-CBDExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
SS80 pEGFPN1 anti-SAPAP2 nanobody
Plasmid#135228PurposeMammalian expression of anti-SAPAP (Rat) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-SAPAP2 (Rat) recombinant llama nanobody SS80 (Dlgap2 L. glama (llama))
UseTags6xHis-HA-EGFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
AC27 pEGFPN1 anti-AMIGO-1 nanobody
Plasmid#135226PurposeMammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-AMIGO-1 (Mouse) recombinant llama nanobody AC27 (Amigo1 L. glama (llama))
UseTags6xHis-HA-EGFPExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJET-41-DExCon-antiGFPnanobody-mCherry-R11b
Plasmid#179901PurposeDonor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsantiGFPnanobody-mCherryExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-42-DExCon-antiGFPnanobody-mCherry-R11a
Plasmid#179902PurposeDonor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry moduleDepositorInsertRAB11A, member RAS oncogene family (RAB11A Human)
UseCRISPR; Donor for homologous based knock inTagsantiGFPnanobody-mCherryExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138133PurposeExpresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertEGFP-anti-rabbit-IgG(Fc)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only