Addgene

We narrowed to 393 results for: PUR ALPHA-1

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shp63alpha pLKO.1 puro

Plasmid

#19120

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against tumor protein p63 (TP63 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2008

Availability

Academic Institutions and Nonprofits only

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pBABE puro SDF-1 alpha

Plasmid

#12270

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

SDF-1 alpha (CXCL12 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pMKO.1-Puro-shPP2A-Aalpha

Plasmid

#15249

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

PP2A regulatory subunit A, alpha isoform (PPP2R1A Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

Plasmid

#128762

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #1 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

Plasmid

#128756

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha-wt-pBabe-puro

Plasmid

#19365

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt human cDNA

Promoter

Available since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha P402A/P564A-pBabe-puro

Plasmid

#19005

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puro

Plasmid

#202800

Purpose

Lentiviral vector to express SVA-lncRNA AK057321 with ires for mcherry and puro expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFalpha (with intron)

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s

Plasmid

#172105

Purpose

Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)-Gαs (GNAS Human)

Use

Tags

Expression

Mammalian

Mutation

Gαs: C3S

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

Plasmid

#128757

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-1 shRev-Erbalpha

Plasmid

#22747

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

shRev-Erbalpha (Nr1d1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only
pLViN-E-cadherin-GFP

Plasmid

#229710

Purpose

Lentiviral expression of E-cadherin-GFPTG in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CDH1 E-CADHERIN Human (CDH1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV p2GM AMPK alpha2hp1 alpha1hp1

Plasmid

#89492

Purpose

shRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expression

Depositor

Russell Jones

Article

Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

Insert

2 hairpins against mouse/human AMPKalpha1 and alpha2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 (The GFP-Puro is from a PGK promoter; the shRN…

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-1

Plasmid

#112841

Purpose

transient/stable overexpression of the N-terminal domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

N domain of Catulin

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-SpGalpha-i

Plasmid

#185568

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

Galpha-i

Use

In vitro transcription (mrna synthesis)

Tags

GFP, S-Tag

Expression

Mutation

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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LMP AMPK alpha1 hp1

Plasmid

#89489

Purpose

shRNA against AMPK alpha1 in mouse/human to knock down protein expression

Depositor

Russell Jones

Article

Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

Insert

hairpin against mouse/human AMPKalpha1 (PRKAA1, Prkaa1 Human, Mouse)

Use

Mouse Targeting and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 (The GFP-Puro is from a PGK promoter; the shRN…

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only
pCAG-hHIF1 alpha-IGd2

Plasmid

#22535

Purpose

To be used for the RNAi screening purposes (not for HDAC4 expression); see paper

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2009

Availability

Academic Institutions and Nonprofits only
LMP AMPK alpha2 hp1

Plasmid

#89490

Purpose

shRNA against AMPK alpha2 in mouse/human to knock down protein expression

Depositor

Russell Jones

Article

Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

Insert

hairpin against mouse/human AMPKalpha2 (PRKAA2, Prkaa2 )

Use

Mouse Targeting and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LMP

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfRabin8_5

Plasmid

#26714

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Dog Rabin8 alpha isoform RNAi

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfRabin8_4

Plasmid

#26713

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

RNAi to dog Rabin8 alpha isoform.

Promoter

Available since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only