Showing: 21 - 36 of 36 results
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Plasmid#97060PurposeExpresses FLAG/HA-tagged DDX54 in mammalian cellsDepositorInsertDDX54 (DDX54 Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/RFP-DDX54
Plasmid#97061PurposeExpresses RFP (tdTomato)-tagged DDX54 in mammalian cellsDepositorInsertDDX54 (DDX54 Human)
UseTagsRFP (tdTomato)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-DDX54-FLAG/HA
Plasmid#97062PurposeExpresses DDX54-FLAG/HA (C-terminal tagged) in mammalian cellsDepositorInsertDDX54 (DDX54 Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/BirA/FLAG-DDX54
Plasmid#97063PurposeExpresses BirA/FLAG-tagged DDX54 in mammalian cellsDepositorInsertDDX54 (DDX54 Human)
UseTagsBirA and FLAGExpressionMammalianMutationPromotertetO2AvailabilityAcademic Institutions and Nonprofits only -
GFP SMG1 WT
Plasmid#199571PurposeExpresses WT SMG1 fused to GFPDepositorInsertSMG1 WT (SMG1 Human)
UseTagsGFP fusion proteinExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
GFP SMG1 T3550A/S3556A
Plasmid#199572PurposeExpresses mutated SMG1 fused to GFPDepositorInsertSMG1 (SMG1 Human)
UseTagsGFPExpressionMammalianMutationT3550A and S3556APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
FLAG SMG1 WT
Plasmid#199573PurposeExpresses FLAG-tagged WT SMG1DepositorInsertSMG1 (SMG1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
FLAG SMG1 T3550A/S3556A
Plasmid#199574PurposeExpresses FLAG-tagged mutated SMG1DepositorInsertSMG1 (SMG1 Human)
UseTagsFLAGExpressionMammalianMutationT3550A and S3556APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
FLAG SMG1 D2335A/N2340K
Plasmid#199575PurposeExpresses FLAG-tagged kinase dead SMG1DepositorInsertSMG1 (SMG1 Human)
UseTagsFLAGExpressionMammalianMutationD2335A and N2340KPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET28sumo-hRig-I DCARDS
Plasmid#210828Purposeprotein expression in ecoliDepositorInserthRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
UseTagsExpressionBacterialMutationNAPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28sumo-hRig-I-Full-length
Plasmid#210827Purposeprotein expression in ecoliDepositorInserthRIG-I (DDX58) Full length protein (RIGI Human)
UseTagsExpressionBacterialMutationNAPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEF-Bos-FLAG RIG-I MIII
Plasmid#52877PurposeExpresses RIG-I with a mutation to motif III in mammalian cellsDepositorInsertDDX58 (RIGI Human)
UseTagsFLAGExpressionMammalianMutationT409A, S411APromoterAvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A RIGI WT
Plasmid#167289PurposeLentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-IDepositorInsertDDX58 (RIGI Human)
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A RIGI K270A
Plasmid#167290PurposeLentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-IDepositorInsertDDX58 (RIGI Human)
UseLentiviralTagstagBFPExpressionMammalianMutationK270APromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pLenti-RIGI-Apex- T2A-EGFP
Plasmid#167288PurposeLentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.DepositorInsertDDX58 (RIGI Human)
UseLentiviralTagsAPEX2 and EGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Alexandrov Cell-Free Protein Expression Kit (PEK)
Plasmid Kit#1000000065PurposeSet of 89 ORFs cloned into the pCellFree vector to enable protein expression in any in vitro translation system; can perform statistically relevant benchmarking of cell-free expression systemsDepositorAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 36 of 36 results