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Showing: 1 - 20 of 234 results

  2. pDSP23

    Plasmid
    #199372
    Purpose
    Encodes dual cistronic nuclear localized GCaMP6s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::GCaMP6s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    199372-plasmid-map-sequence-id-390928

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  3. pDSP31

    Plasmid
    #199373
    Purpose
    Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    199373-plasmid-map-sequence-id-390590

    Enlarge

  4. pDSP32

    Plasmid
    #199374
    Purpose
    Encodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    199374-plasmid-map-sequence-id-390801

    Enlarge

  5. pDSP33

    Plasmid
    #199375
    Purpose
    Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    199375-plasmid-map-sequence-id-391013

    Enlarge

  6. pDSP1

    Plasmid
    #199384
    Purpose
    Kanamycin resistant cloning vector backbone for pDSP23, pDSP31-33, pHJL1-4, pSiM1-4
    Depositor
    Hang Lu
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cloning vector backbone
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    199384-plasmid-map-sequence-id-390594

    Enlarge

  9. dSpCas9-VPR

    Plasmid
    #205247
    Purpose
    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.
    Depositor
    Rasmus Bak
    Article
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSpCas9-VPR
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    205247-plasmid-map-sequence-id-408440

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  10. KRAB-dSpCas9

    Plasmid
    #205248
    Purpose
    In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.
    Depositor
    Rasmus Bak
    Article
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    KRAB-dSpCas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    205248-plasmid-map-sequence-id-409108

    Enlarge

  11. pYPQ-dSpRY-Act3.0

    Plasmid
    #158416
    Purpose
    CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    158416-plasmid-map-sequence-id-337116

    Enlarge

  17. dSp-p65 (1-200)

    Plasmid
    #99658
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Insert
    dCas9
    Use
    Tags
    truncated p65 activation domain (1-200)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99658-plasmid-map-sequence-id-191936

    Enlarge

  18. dSp-p65 (1-150)

    Plasmid
    #99659
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Insert
    dCas9
    Use
    Tags
    truncated p65 activation domain (1-150)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99659-plasmid-map-sequence-id-191939

    Enlarge

  19. dSp-p65 (1-100)

    Plasmid
    #99660
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Insert
    dCas9
    Use
    Tags
    truncated p65 activation domain (1-100)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99660-plasmid-map-sequence-id-189821

    Enlarge

  20. dSp-p65 (50-150)

    Plasmid
    #99661
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Insert
    dCas9
    Use
    Tags
    truncated p65 activation domain (50-150)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99661-plasmid-map-sequence-id-189830

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Showing: 1 - 20 of 234 results