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SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library E484K Tile 1 (positions 333-436)

Pooled Library

#174293

Purpose

This library contains mutations to SARS-CoV-2 Spike RBD E484K variant in which all surface exposed residues on S RBD (Tile 1, positions 333-436) were mutated to every other amino acid.

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Yeast

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library E484K Tile 2 (positions 437-527)

Pooled Library

#174294

Purpose

This library contains mutations to SARS-CoV-2 Spike RBD E484K variant in which all surface exposed residues on S RBD (Tile 2, positions 437-527) were mutated to every other amino acid.

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Yeast

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library N501Y Tile 1 (positions 333-436)

Pooled Library

#174295

Purpose

This library contains mutations to SARS-CoV-2 Spike RBD N501Y variant in which all surface exposed residues on S RBD (Tile 1, positions 333-436) were mutated to every other amino acid.

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Yeast

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library N501Y Tile 2 (positions 437-527)

Pooled Library

#174296

Purpose

This library contains mutations to SARS-CoV-2 Spike RBD N501Y variant in which all surface exposed residues on S RBD (Tile 2, positions 437-527) were mutated to every other amino acid.

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Yeast

Availability

Academic Institutions and Nonprofits only
pcDNA3 hE47

Plasmid

#16059

Depositor

Robert Benezra

Article

Jen et al Genes Dev. 1992 Aug . 6(8):1466-79.

Insert

E47 (TCF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pNTI827

Plasmid

#216635

Purpose

Cal-ID-ERM (V5) ER membrane-localized Cal-ID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol (2024)

Insert

Cal-ID-ERM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pNTI934

Plasmid

#216637

Purpose

Cal-ID-PM (V5) Plasma membrane-localized Cal-ID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol (2024)

Insert

Cal-ID-PM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRSpuro shID1#6

Plasmid

#19165

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 1 (ID1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
EFS-mTSG

Pooled Library

#113584

Purpose

AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes

Depositor

Sidi Chen

Article

Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

Expression

Mammalian

Use

AAV, CRISPR, Lentiviral, and Luciferase

Availability

Academic Institutions and Nonprofits only
TBG-mTSG

Pooled Library

#113585

Purpose

AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes in mouse liver

Depositor

Sidi Chen

Article

Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

Expression

Mammalian

Use

AAV, CRISPR, Lentiviral, and Luciferase

Availability

Academic Institutions and Nonprofits only
Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library

Pooled Library

#138015

Purpose

This library can be used to investigate cardiac gene regulation. It targets transcriptional regulators that are expressed in mouse cardiomyocytes. A subset of GATA4 regulated genes are also included.

Depositor

William Pu

Article

VanDusen et al bioRxiv 808402v1 https://www.biorxiv.or

Species

Mus musculus

Use

AAV, CRISPR, and Cre/Lox

Availability

Academic Institutions and Nonprofits only
-4200GAMT-P1-pGL4

Plasmid

#26804

Depositor

Sam Lee

Article

Ide et al Mol Cell. 2009 Nov 13. 36(3):379-92.

Insert

Guanidinoacetate N-methyltransferase (GAMT Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

Plasmid

#208038

Purpose

Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin

Depositor

James Sutherland, Rosa Barrio

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

PML (isoform IVa) (PML Human)

Use

Lentiviral

Tags

FLAG, N-terminal Split-TurboID

Expression

Mutation

Promoter

EFS

Availability

Academic Institutions and Nonprofits only

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2PLU

Plasmid

#60131

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

cgd2_4120:MAC01Y-E08:C33348

Use

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Availability

Academic Institutions and Nonprofits only
4GM2

Plasmid

#60132

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

PF14_0348:PF14_0348_dml-X03:C228094

Use

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Availability

Academic Institutions and Nonprofits only
2I7C

Plasmid

#60124

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

PY03671:MAC01H-E01:C21952

Use

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Availability

Academic Institutions and Nonprofits only
pet21_TurboID_18A

Plasmid

#170922

Purpose

Express TurboID_18A in Bl21

Depositor

Huan Bao

Article

Bao et al Commun Biol. 2021 Apr 30;4(1):514. doi:

Insert

TurboID-18a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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28A_APEX2_MSP1D1

Plasmid

#170923

Purpose

Express APEX2_MSP1D1 in Bl21

Depositor

Huan Bao

Article

Bao et al Commun Biol. 2021 Apr 30;4(1):514. doi:

Insert

APEX2-MSP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pNTI819

Plasmid

#216628

Purpose

Cal-ID (V5) for mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol (2024)

Insert

Cal-ID

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
FEM1C

Plasmid

#167842

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

FEM1C (FEM1C Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge