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Addgene
Showing: 41 - 60 of 6549 results

  1. pORTMAGE502B

    Plasmid
    #128971
    Purpose
    Broad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    xyl-Pm
    Availability
    Academic Institutions and Nonprofits only
    128971-plasmid-map-sequence-id-252654

    Enlarge

  2. pORTMAGE311B

    Plasmid
    #120418
    Purpose
    Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR
    Depositor
    Csaba Pál
    Article
    Szili et al bioRxiv (2018)
    Inserts
    MutL E32K
    Lambda Beta
    XylS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Pm
    Availability
    Academic Institutions and Nonprofits only
    120418-plasmid-map-sequence-id-236013

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  5. pORTMAGE-2

    Plasmid
    #72677
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    pL promoter
    Availability
    Academic Institutions and Nonprofits only
    72677-plasmid-map-sequence-id-133224

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  6. pORTMAGE-3

    Plasmid
    #72678
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    72678-plasmid-map-sequence-id-198909

    Enlarge

  7. pORTMAGE-4

    Plasmid
    #72679
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    72679-plasmid-map-sequence-id-198908

    Enlarge

  8. pORTMAGE-1

    Plasmid
    #72680
    Purpose
    Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    lower strength RBS before mutL E32K allele than i…
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  9. MAGEA3

    Plasmid
    #73246
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAGEA3 (MAGEA3 Human)
    Use
    Tags
    His6-Thioredoxin-TEV
    Expression
    Bacterial
    Mutation
    pfam01454:MAGE 116-286
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    73246-plasmid-map-sequence-id-137200

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  12. p41Nat MagicFluor

    Plasmid
    #58563
    Purpose
    p41Nat carrying both mating type markers buffered by the Cyc1 terminator
    Depositor
    Leonid Kruglyak
    Insert
    Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    58563-plasmid-map-sequence-id-90668

    Enlarge

  13. p41Neo MagicFluor

    Plasmid
    #58564
    Purpose
    p41Neo carrying both mating type markers buffered by the Cyc1 terminator
    Depositor
    Leonid Kruglyak
    Insert
    Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    58564-plasmid-map-sequence-id-90669

    Enlarge

Showing: 41 - 60 of 6549 results