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Addgene
Showing: 41 - 60 of 1727 results

  1. pAM5081

    Plasmid
    #85107
    Purpose
    Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    YFP-KaiC
    Use
    Tags
    YFP
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    85107-plasmid-map-sequence-id-180104

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  2. pAM5082

    Plasmid
    #85121
    Purpose
    PkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization exp
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    PkaiB-KaiC-ECFP
    Use
    Tags
    ECFP
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    85121-plasmid-map-sequence-id-180106

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  3. pAM5085

    Plasmid
    #85122
    Purpose
    PkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
    Use
    Tags
    ECFP
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    85122-plasmid-map-sequence-id-180108

    Enlarge

  9. pAM002

    Plasmid
    #170006
    Purpose
    Encodes PpmrD-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1 and PpmrD
    Availability
    Academic Institutions and Nonprofits only
    170006-plasmid-map-sequence-id-335939

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  10. pAM003

    Plasmid
    #170007
    Purpose
    Encodes PmgtC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1 and PmgtC
    Availability
    Academic Institutions and Nonprofits only
    170007-plasmid-map-sequence-id-335948

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  11. pAM004

    Plasmid
    #170008
    Purpose
    Encodes PugtL-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1 and PugtL
    Availability
    Academic Institutions and Nonprofits only
    170008-plasmid-map-sequence-id-335294

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  12. pAM005

    Plasmid
    #170009
    Purpose
    Encodes PvirK-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1 and PvirK
    Availability
    Academic Institutions and Nonprofits only
    170009-plasmid-map-sequence-id-335956

    Enlarge

  13. pAM006

    Plasmid
    #170010
    Purpose
    Encodes PpagC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1 and PpagC
    Availability
    Academic Institutions and Nonprofits only
    170010-plasmid-map-sequence-id-335938

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  14. pAM007

    Plasmid
    #170011
    Purpose
    Encodes PslyB-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1 and PslyB
    Availability
    Academic Institutions and Nonprofits only
    170011-plasmid-map-sequence-id-335739

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  20. pRCPam

    Plasmid
    #195861
    Purpose
    Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Insert
    Amber Chromogenic Protein on pRCPam plasmid
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Gln62X
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    195861-plasmid-map-sequence-id-384172

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Showing: 41 - 60 of 1727 results