Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 355 results for: plasmid transfection

Showing: 1 - 20 of 355 results

  4. pSFV3

    Plasmid
    #92072
    Purpose
    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.
    Depositor
    Henrik Garoff
    Article
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Type
    Empty backbone
    Use
    Empty expression plasmid, for run-off transcripti…
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92072-plasmid-map-sequence-id-181621

    Enlarge

  5. pONSY-mCherry

    Plasmid
    #111874
    Purpose
    This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111874-plasmid-map-sequence-id-213482

    Enlarge

  6. pONSY-Venus

    Plasmid
    #111875
    Purpose
    This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Venus
    Use
    Capsaspora owczarzaki
    Tags
    Venus
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111875-plasmid-map-sequence-id-213485

    Enlarge

  7. pONSY-CoH2B:Venus

    Plasmid
    #111877
    Purpose
    This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Capsaspora Histone H2B (CoH2B) fused to Venus
    Use
    Capsaspora owczarzaki
    Tags
    Venus
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111877-plasmid-map-sequence-id-213489

    Enlarge

  9. pDOE20 mC VN transient

    Plasmid
    #124247
    Purpose
    Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in directly-transfected plant cells.
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124247-plasmid-map-sequence-id-239326

    Enlarge

  10. pTM

    Plasmid
    #162783
    Purpose
    Protein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are coded
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Type
    Empty backbone
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Promoter
    CHO Elongation factor 1A
    Available Since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162783-plasmid-map-sequence-id-333802

    Enlarge

  11. pBD21_037

    Plasmid
    #109154
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    iRFP720
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109154-plasmid-map-sequence-id-209237

    Enlarge

  12. pBD21_033

    Plasmid
    #109150
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of Sirius
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    Sirius
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109150-plasmid-map-sequence-id-209216

    Enlarge

  13. pBD21_036

    Plasmid
    #109153
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    mKO2
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109153-plasmid-map-sequence-id-209235

    Enlarge

  14. pBD21_034

    Plasmid
    #109151
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFP
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    TagBFP
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    109151-plasmid-map-sequence-id-220104

    Enlarge

  15. pBD21_035

    Plasmid
    #109152
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFP
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    EGFP
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109152-plasmid-map-sequence-id-209236

    Enlarge

  16. CMV-pDisplay-Halo-T2A-EGFP

    Plasmid
    #223498
    Purpose
    Co-expression of HaloTags to outer surface in mammalian cells coupled with a EGFP transfection marker
    Depositor
    James Frank
    Insert
    HaloTag and EGFP
    Expression
    Mammalian
    Available Since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    223498-plasmid-map-sequence-id-443020

    Enlarge

  18. hSyn-pDisplay-SNAP-T2A-EGFP

    Plasmid
    #223503
    Purpose
    Co-expression of SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker
    Depositor
    James Frank
    Insert
    SNAP-tag and EGFP
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223503-plasmid-map-sequence-id-443301

    Enlarge

  20. p1.1-Tr2-eGFP

    Plasmid
    #162782
    Purpose
    Fluorescent reporter for protein expression studies
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    162782-plasmid-map-sequence-id-338334

    Enlarge

Showing: 1 - 20 of 355 results