We narrowed to 344 results for: plasmid transfection

1. pEXP1/Joseph2-Multitagged Plasmid

   Plasmid

   #226724

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in bacterial cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pEXP1/Joseph2
   

   Use

   Tags

   6xHis, HA, Myc, GFP, Flag, V5

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMH-HA/Joseph2-Multitagged Plasmid

   Plasmid

   #226726

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pMH-HA/Joseph2
   

   Use

   Tags

   HA, Myc, GFP, Flag, V5, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAHW/Joseph2-Multitagged Plasmid

   Plasmid

   #226725

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in Drosophila cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pAHW/Joseph2
   

   Use

   Drosophila s2 cell expression

   Tags

   HA, Myc, GFP, Flag, V5, 6xHis

   Expression

   Insect

   Mutation

   Promoter

   Act5C

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSFV3

   Plasmid

   #92072

   Purpose

   Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

   Depositor

   Henrik Garoff

   Article

   Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

   Type

   Empty backbone

   Use

   Empty expression plasmid, for run-off transcripti…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pONSY-mCherry

   Plasmid

   #111874

   Purpose

   This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   mCherry
   

   Use

   Capsaspora owczarzaki

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pONSY-Venus

   Plasmid

   #111875

   Purpose

   This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Venus
   

   Use

   Capsaspora owczarzaki

   Tags

   Venus

   Expression

   Mutation

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pONSY-CoH2B:Venus

   Plasmid

   #111877

   Purpose

   This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Capsaspora Histone H2B (CoH2B) fused to Venus
   

   Use

   Capsaspora owczarzaki

   Tags

   Venus

   Expression

   Mutation

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PM-GFP

   Plasmid

   #21213

   Depositor

   Tobias Meyer

   Article

   Teruel et al J Neurosci Methods. 1999 Oct 30. 93(1):3

   Insert

   PM-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PM targetting sequence is Lyn (MGCIKSKRKD) (Lab …

   Promoter

   Available since

   July 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDOE20 mC VN transient

   Plasmid

   #124247

   Purpose

   Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in directly-transfected plant cells.

   Depositor

   Attila Fehér

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTM

    Plasmid

    #162783

    Purpose

    Protein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are coded

    Depositor

    Ivan Vorobiev

    Article

    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

    Type

    Empty backbone

    Use

    Tags

    10xHis, c-myc, and hTPA leader

    Expression

    Mammalian

    Mutation

    Promoter

    CHO Elongation factor 1A

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBD21_037

    Plasmid

    #109154

    Purpose

    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720

    Depositor

    Ron Weiss

    Article

    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

    Inserts

    mRFP
    iRFP720
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and pTet

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBD21_033

    Plasmid

    #109150

    Purpose

    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of Sirius

    Depositor

    Ron Weiss

    Article

    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

    Inserts

    mRFP
    Sirius
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and pTet

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBD21_036

    Plasmid

    #109153

    Purpose

    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2

    Depositor

    Ron Weiss

    Article

    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

    Inserts

    mRFP
    mKO2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and pTet

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBD21_034

    Plasmid

    #109151

    Purpose

    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFP

    Depositor

    Ron Weiss

    Article

    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

    Inserts

    mRFP
    TagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and pTet

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBD21_035

    Plasmid

    #109152

    Purpose

    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFP

    Depositor

    Ron Weiss

    Article

    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

    Inserts

    mRFP
    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and pTet

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-pDisplay-Halo-T2A-EGFP

    Plasmid

    #223498

    Purpose

    Co-expression of HaloTags to outer surface in mammalian cells coupled with a EGFP transfection marker

    Depositor

    James Frank

    Insert

    HaloTag and EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. hSyn-pDisplay-SNAP-T2A-EGFP

    Plasmid

    #223503

    Purpose

    Co-expression of SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker

    Depositor

    James Frank

    Insert

    SNAP-tag and EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGFP-LacI

    Plasmid

    #179510

    Purpose

    Expresses EGFP-LacI in mammalian cells

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    LacI
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p1.1-Tr2-eGFP

    Plasmid

    #162782

    Purpose

    Fluorescent reporter for protein expression studies

    Depositor

    Ivan Vorobiev

    Article

    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

    Insert

    enhanced green fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. hsLEMD2-pX330

    Plasmid

    #179511

    Purpose

    Encodes gRNA for human LEMD2

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    gRNA for LEMD2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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