Addgene

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IF189

Bacterial Strain

#134836

Purpose

Rapid and efficient recombineering and purificiation of modified lambda phage DNA

Depositor

IIya Finkelstein

Article

Kim et al Sci Rep. 2017 May 18;7(1):2071. doi: 10.

Bacterial Resistance

None

Available since

Jan. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits
E. coli K-12 MG1655_rLP5(nth-ydgR)

Bacterial Strain

#110245

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
C321.ΔClpS.Ub-UAG-sfGFP

Bacterial Strain

#98565

Purpose

Recoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter and ClpS inactivated

Depositor

George Church

Article

Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

Bacterial Resistance

Ampicillin

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP4(essQ-cspB)

Bacterial Strain

#110244

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP6(ygcE-ygcF)

Bacterial Strain

#110246

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the ygcE-ygcF intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP1(atpI-gidB)

Bacterial Strain

#110241

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the atpl-gidB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP3(ybbD-ylbG)

Bacterial Strain

#110243

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the ybbD-ylbG intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP2(yieN-trkD)

Bacterial Strain

#110242

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the yieN-trkD intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
HL2483

Bacterial Strain

#62819

Purpose

Lim Lab strain: MG1655 + lacIq intergrated at intS + Pcon::TetR inserted into the chromosome at galK + ΔglgCAP + ΔcsrB + ΔcsrC + ΔcsrD

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Bacterial Resistance

None

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
V. natriegens NC7

Bacterial Strain

#215356

Purpose

Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

Depositor

Buz Barstow

Bacterial Resistance

None

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only
V. natriegens NC1

Bacterial Strain

#215355

Purpose

Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.

Depositor

Buz Barstow

Bacterial Resistance

None

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only
Strain SIJ_488

Bacterial Strain

#68246

Purpose

E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.

Depositor

Alex Nielsen

Article

Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

Bacterial Resistance

None

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 ΔrecBCD GamS

Bacterial Strain

#176586

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol and ampicillin

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
C321.∆A.opt

Bacterial Strain

#87359

Purpose

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

Depositor

George Church

Article

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

Bacterial Resistance

Gentamicin

Species

Synthetic

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 GamS

Bacterial Strain

#176584

Purpose

E. coli BL21 strain for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol and ampicillin

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 ΔrecB GamS

Bacterial Strain

#176585

Purpose

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol and ampicillin

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
C321.deltaA (Isaacs lab)

Bacterial Strain

#73581

Purpose

Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Bacterial Resistance

Ampicillin

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
BM28

Bacterial Strain

#201047

Purpose

MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures. Also carries a pBR325 plasmid carrying groESL called pOF39.

Depositor

Jeannette Winter

Article

McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi

Bacterial Resistance

Ampicillin and kanamycin

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only
RF12

Bacterial Strain

#62077

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. trpA trpB genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
ML2

Bacterial Strain

#61906

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. ilvE gene deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

Kanamycin

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

Showing: 41 - 60 of 131 results

Results per page:

Sort results by:

IF189

E. coli K-12 MG1655_rLP5(nth-ydgR)

C321.ΔClpS.Ub-UAG-sfGFP

E. coli K-12 MG1655_fLP4(essQ-cspB)

E. coli K-12 MG1655_rLP6(ygcE-ygcF)

E. coli K-12 MG1655_rLP1(atpI-gidB)

E. coli K-12 MG1655_fLP3(ybbD-ylbG)

E. coli K-12 MG1655_fLP2(yieN-trkD)

HL2483

V. natriegens NC7

V. natriegens NC1

Strain SIJ_488

BL21 Rosetta2 ΔrecBCD GamS

C321.∆A.opt

BL21 Rosetta2 GamS

BL21 Rosetta2 ΔrecB GamS

C321.deltaA (Isaacs lab)

BM28

RF12

ML2

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