422 results

1. BL21(DE3) ΔserC

   Bacterial Strain

   #197656

   Purpose

   Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

   Depositor

   Ryan Mehl, Richard Cooley

   Article

   Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

   Bacterial Resistance

   None

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

2. USO hisB- pyrF- rpoZ-

   Bacterial Strain

   #18049

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

   Bacterial Resistance

   Tetracycline

   Available Since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

3. C321.ΔA.M9adapted

   Bacterial Strain

   #98568

   Purpose

   Recoded strain C321.ΔA adaptively evolved for 1000+ generations in M9 minimal media + 1% glucose and biotin

   Depositor

   George Church

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

   Bacterial Resistance

   Ampicillin

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

4. TB205

   Bacterial Strain

   #230034

   Purpose

   Fluorescently labelled (mCherry) E. coli

   Depositor

   Calin Guet, Tobias Bergmiller

   Article

   Bergmiller et al Science. 2017 Apr 21;356(6335):311-315.

   Bacterial Resistance

   None

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

5. C321.∆A.opt

   Bacterial Strain

   #87359

   Purpose

   This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

   Depositor

   George Church

   Article

   Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

   Bacterial Resistance

   Gentamicin

   Species

   Synthetic

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

6. Syn61Δ3(ev5) ΔrecA (ev1)

   Bacterial Strain

   #189857

   Purpose

   Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

   Depositor

   George Church

   Article

   Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

   Bacterial Resistance

   Streptomycin

   Species

   Synthetic

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

7. DHIP1

   Bacterial Strain

   #226851

   Purpose

   Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

   Depositor

   David Nielsen

   Article

   Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

   Bacterial Resistance

   Streptomycin

   Species

   Escherichia coli

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

8. FW102 OL2-62

   Bacterial Strain

   #53735

   Purpose

   Bacterial-two hybrid reporter strain

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Bacterial Resistance

   Kanamycin and streptomycin

   Species

   Escherichia coli

   Available Since

   June 18, 2014

   Availability

   Academic Institutions and Nonprofits only

9. BL21∆serB

   Bacterial Strain

   #34929

   Depositor

   Jesse Rinehart, Dieter Söll

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Bacterial Resistance

   None

   Available Since

   Feb. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

10. MW005 (bacterial strain)

    Bacterial Strain

    #24545

    Depositor

    Colin Dolphin

    Article

    Westenberg et al BMC Biotechnol. 2010 Mar 29. 10(1):27.

    Bacterial Resistance

    Streptomycin

    Available Since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

11. CJW4617

    Bacterial Strain

    #177118

    Purpose

    E. coli strain carrying IPTG-inducible gfp-muNS gene for monitoring the mobility of cytoplasmic particles.

    Depositor

    Christine Jacobs-Wagner

    Article

    Parry et al Cell. 2014 Jan 16;156(1-2):183-94. doi:

    Bacterial Resistance

    Kanamycin

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

12. MG1655 ΔendA ΔrecA (DE3)

    Bacterial Strain

    #37854

    Depositor

    Kristala Prather

    Article

    Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

    Bacterial Resistance

    None

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

13. JM109

    Bacterial Strain

    #49761

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Yanisch-Perron et al Gene. 1985;33(1):103-19.

    Bacterial Resistance

    None

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

14. sAJM.1506

    Bacterial Strain

    #108254

    Purpose

    Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Bacterial Resistance

    Chloramphenicol

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

15. Strain SIJ_488

    Bacterial Strain

    #68246

    Purpose

    E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Bacterial Resistance

    None

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

16. RF4

    Bacterial Strain

    #62072

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB genes deleted.

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

17. E. coli BL21 (DE3) ΔEntD

    Bacterial Strain

    #192874

    Purpose

    Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

    Depositor

    Christophe Guilhot, Christian Chalut

    Article

    Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

18. Syn61

    Bacterial Strain

    #174513

    Purpose

    Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

    Depositor

    Jason W Chin

    Article

    Fredens et al Nature. 2019 May;569(7757):514-518. doi:

    Bacterial Resistance

    Streptomycin

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

19. BL21 ΔrecBCD

    Bacterial Strain

    #176581

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

    Depositor

    Jean-Loup Faulon, Chase Beisel

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    None

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

20. LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

    Bacterial Strain

    #99247

    Purpose

    LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

    Depositor

    Jeff Hasty

    Article

    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

    Bacterial Resistance

    Tetracycline

    Available Since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only