425 results

1. BL21(DE3)-R3-pRARE2

   Bacterial Strain

   #26242

   Purpose

   Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Bacterial Resistance

   Chloramphenicol

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

2. DHIP1

   Bacterial Strain

   #226851

   Purpose

   Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

   Depositor

   David Nielsen

   Article

   Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

   Bacterial Resistance

   Streptomycin

   Species

   Escherichia coli

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

3. Syn61Δ3(ev5)

   Bacterial Strain

   #174514

   Purpose

   Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Bacterial Resistance

   Streptomycin

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

4. LC-E75

   Bacterial Strain

   #115925

   Purpose

   Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Bacterial Resistance

   None

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

5. E. coli ER1821ΔlacI

   Bacterial Strain

   #141407

   Purpose

   LacI-deficient E. coli host bacterium that produces β-galactosidase constitutively

   Depositor

   Antal Kiss

   Article

   Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

   Bacterial Resistance

   None

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

6. E. coli BL21 (DE3) ΔEntD

   Bacterial Strain

   #192874

   Purpose

   Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

   Depositor

   Christian Chalut, Christophe Guilhot

   Article

   Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

7. MG1655 ΔendA ΔrecA

   Bacterial Strain

   #37853

   Depositor

   Kristala Prather

   Article

   Leonard et al Proc Natl Acad Sci U S A. 2010 Aug 3;107

   Bacterial Resistance

   None

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

8. sAJM.1506

   Bacterial Strain

   #108254

   Purpose

   Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Bacterial Resistance

   Chloramphenicol

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

9. C321.∆A.opt

   Bacterial Strain

   #87359

   Purpose

   This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

   Depositor

   George Church

   Article

   Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

   Bacterial Resistance

   Gentamicin

   Species

   Synthetic

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

10. S1030

    Bacterial Strain

    #105063

    Purpose

    host strain for phage assisted continuous evolution

    Depositor

    David Liu

    Article

    Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

    Bacterial Resistance

    Streptomycin and Tetracycline

    Available Since

    March 21, 2018

    Availability

    Academic Institutions and Nonprofits only

11. rEcoli XpS

    Bacterial Strain

    #192872

    Purpose

    MG1655-C321 -- All 321 UAG codons changed to UAA

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

    Bacterial Resistance

    Bleocin (Zeocin)

    Species

    Escherichia coli

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

12. ATMW-BL21

    Bacterial Strain

    #218769

    Purpose

    E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

    Depositor

    Abhishek Chatterjee

    Article

    Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

    Bacterial Resistance

    Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)

    Species

    Escherichia coli

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

13. C321.∆A ompT- rne- endA- lon-

    Bacterial Strain

    #182775

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Ampicillin

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

14. C321.deltaA (Isaacs lab)

    Bacterial Strain

    #73581

    Purpose

    Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Bacterial Resistance

    Ampicillin

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

15. DH10B-ALT

    Bacterial Strain

    #61151

    Purpose

    DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Bacterial Resistance

    None

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

16. ATMY-C321

    Bacterial Strain

    #218774

    Purpose

    E. coli strain, genotype: C321.ΔA pUltraBR-MjY ΔtyrS tyrTV::tolC tyrU::GentR lambda::ZeoR

    Depositor

    Abhishek Chatterjee

    Article

    Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

    Bacterial Resistance

    Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)

    Species

    Escherichia coli

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

17. Syn61Δ3(ev5) ΔrecA (ev1)

    Bacterial Strain

    #189857

    Purpose

    Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

    Depositor

    George Church

    Article

    Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

    Bacterial Resistance

    Streptomycin

    Species

    Synthetic

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

18. MG1655 Z1 malE

    Bacterial Strain

    #65915

    Purpose

    E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Bacterial Resistance

    None

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

19. EC1000

    Bacterial Strain

    #71852

    Purpose

    RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

    Depositor

    Todd Klaenhammer, Jan Kok

    Article

    Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

    Bacterial Resistance

    Kanamycin

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

20. DHL708

    Bacterial Strain

    #98417

    Purpose

    Bacterial Strain with Genotype: MC4100 ∆clpPX

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Bacterial Resistance

    Streptomycin

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only