422 results

1. BL21ΔABCF

   Bacterial Strain

   #102270

   Purpose

   ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

   Depositor

   Jack Leo

   Article

   Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

   Bacterial Resistance

   Tetracycline

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

2. V. natriegens NC1

   Bacterial Strain

   #215355

   Purpose

   Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.

   Depositor

   Buz Barstow

   Article

   Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

   Bacterial Resistance

   None

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

3. ATMW-BL21

   Bacterial Strain

   #218769

   Purpose

   E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

   Depositor

   Abhishek Chatterjee

   Article

   Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

   Bacterial Resistance

   Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

   Species

   Escherichia coli

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

4. Syn61Δ3(ev5) ΔrecA (ev1)

   Bacterial Strain

   #189857

   Purpose

   Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

   Depositor

   George Church

   Article

   Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

   Bacterial Resistance

   Streptomycin

   Species

   Synthetic

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

5. rEcoli XpS

   Bacterial Strain

   #192872

   Purpose

   MG1655-C321 -- All 321 UAG codons changed to UAA

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

   Bacterial Resistance

   Bleocin (zeocin)

   Species

   Escherichia coli

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

6. C321.∆A.opt

   Bacterial Strain

   #87359

   Purpose

   This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

   Depositor

   George Church

   Article

   Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

   Bacterial Resistance

   Gentamicin

   Species

   Synthetic

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

7. C321.∆A T7RNAP ompT- rne-

   Bacterial Strain

   #182777

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Spectinomycin

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

8. E. coli MEV20

   Bacterial Strain

   #197113

   Purpose

   E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Bacterial Resistance

   None

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

9. E. coli BW25113 ΔtnaA ΔtrpR

   Bacterial Strain

   #205015

   Purpose

   Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

   Depositor

   Yajun Yan

   Article

   Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available since

   Aug. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

10. JM83

    Bacterial Strain

    #50348

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Messing et al Recombinant DNA Technical Bulletin, 1979

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

11. DH5alpha::DddI

    Bacterial Strain

    #179631

    Purpose

    For expression of toxic DddA

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Bacterial Resistance

    Gentamicin

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

12. TB204 △metA

    Bacterial Strain

    #230039

    Purpose

    Fluorescently labelled (GFP) E. coli, auxotrophic for methionine

    Depositor

    Olga Schubert, Martin Ackermann

    Article

    Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

13. DHIP1

    Bacterial Strain

    #226851

    Purpose

    Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

    Depositor

    David Nielsen

    Article

    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

    Bacterial Resistance

    Streptomycin

    Species

    Escherichia coli

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

14. MG1655GFP

    Bacterial Strain

    #109393

    Purpose

    MG1655 with a "capacity monitor" inserted in the lambda phage integration site.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Bacterial Resistance

    Kanamycin

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

15. RF13

    Bacterial Strain

    #62078

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB genes deleted.

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

16. nuc5-

    Bacterial Strain

    #40804

    Depositor

    George Church

    Article

    Mosberg et al PLoS One. 2012;7(9):e44638. Epub 2012 Se

    Bacterial Resistance

    Ampicillin

    Available since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. BL21ΔBCF

    Bacterial Strain

    #102269

    Purpose

    lamB, ompC, ompF triple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

18. CJW4617

    Bacterial Strain

    #177118

    Purpose

    E. coli strain carrying IPTG-inducible gfp-muNS gene for monitoring the mobility of cytoplasmic particles.

    Depositor

    Christine Jacobs-Wagner

    Article

    Parry et al Cell. 2014 Jan 16;156(1-2):183-94. doi:

    Bacterial Resistance

    Kanamycin

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

19. ∆cosσε ∆HG

    Bacterial Strain

    #197132

    Purpose

    Deficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Bacterial Resistance

    Hygromycin

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

20. BL21 Rosetta2 ΔrecBCD GamS

    Bacterial Strain

    #176586

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only