423 results

1. Syn61Δ3(ev5)

   Bacterial Strain

   #174514

   Purpose

   Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Bacterial Resistance

   Streptomycin

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

2. RF1

   Bacterial Strain

   #61962

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. glyA gene deleted

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

3. E. coli BL21 (DE3) ΔEntD

   Bacterial Strain

   #192874

   Purpose

   Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

   Depositor

   Christophe Guilhot, Christian Chalut

   Article

   Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

4. RF2

   Bacterial Strain

   #62070

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC gene deleted

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

5. Syn61

   Bacterial Strain

   #174513

   Purpose

   Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

   Depositor

   Jason W Chin

   Article

   Fredens et al Nature. 2019 May;569(7757):514-518. doi:

   Bacterial Resistance

   Streptomycin

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

6. V. natriegens NC7

   Bacterial Strain

   #215356

   Purpose

   Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

   Depositor

   Buz Barstow

   Article

   Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

   Bacterial Resistance

   None

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

7. S1030

   Bacterial Strain

   #105063

   Purpose

   host strain for phage assisted continuous evolution

   Depositor

   David Liu

   Article

   Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

   Bacterial Resistance

   Streptomycin and tetracycline

   Available Since

   March 21, 2018

   Availability

   Academic Institutions and Nonprofits only

8. MG1655 Z1 malE

   Bacterial Strain

   #65915

   Purpose

   E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

   Depositor

   Keith Tyo

   Article

   Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

   Bacterial Resistance

   None

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

9. EC1000

   Bacterial Strain

   #71852

   Purpose

   RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

   Depositor

   Jan Kok, Todd Klaenhammer

   Article

   Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

   Bacterial Resistance

   Kanamycin

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

10. BL21ΔABCF

    Bacterial Strain

    #102270

    Purpose

    ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    Tetracycline

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pRK24

    Bacterial Strain

    #51950

    Purpose

    broad-host range natural conjugative plasmid

    Depositor

    Farren Isaacs

    Article

    Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

    Bacterial Resistance

    Tetracycline

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

12. MutaT7

    Bacterial Strain

    #156460

    Purpose

    An E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Bacterial Resistance

    Streptomycin

    Species

    Rattus norvegicus

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

13. C321.ΔA

    Bacterial Strain

    #48998

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Ampicillin

    Species

    Escherichia coli

    Available Since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

14. HB101 endA::frt lambda pir

    Bacterial Strain

    #45472

    Depositor

    Michele Swanson

    Article

    Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

    Bacterial Resistance

    Streptomycin

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

15. bMS.346

    Bacterial Strain

    #220588

    Purpose

    Increase editing efficiency of retron and oligo recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Bacterial Resistance

    None

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

16. ML14

    Bacterial Strain

    #61911

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. tyrA gene deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

    Bacterial Resistance

    None

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

17. V. natriegens NC1

    Bacterial Strain

    #215355

    Purpose

    Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.

    Depositor

    Buz Barstow

    Article

    Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

    Bacterial Resistance

    None

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

18. eScaf

    Bacterial Strain

    #220921

    Purpose

    cssDNA production strain

    Depositor

    Shawn Douglas

    Article

    Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

19. DH10B-M.Osp807II

    Bacterial Strain

    #114267

    Purpose

    The strain expresses M.Osp807II methylase and zeocin selection marker from the arsB locus.

    Depositor

    Christopher A. O'Callaghan

    Article

    Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

    Bacterial Resistance

    Bleocin (zeocin)

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

20. BL21(DE3) ΔserC

    Bacterial Strain

    #197656

    Purpose

    Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Bacterial Resistance

    None

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only