423 results

1. AdEasier-1 cells (strain)

   Bacterial Strain

   #16399

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Bacterial Resistance

   Ampicillin and streptomycin

   Species

   Escherichia coli

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

2. LC-E75

   Bacterial Strain

   #115925

   Purpose

   Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Bacterial Resistance

   None

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

3. B-95.ΔAΔfabR

   Bacterial Strain

   #197934

   Purpose

   BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

   Depositor

   Kensaku Sakamoto

   Article

   Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

   Bacterial Resistance

   None

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

4. B-95.ΔA

   Bacterial Strain

   #197933

   Purpose

   BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

   Depositor

   Kensaku Sakamoto

   Article

   Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

   Bacterial Resistance

   None

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

5. TB204

   Bacterial Strain

   #230033

   Purpose

   Fluorescently labelled (monomeric sfGFP) E. coli

   Depositor

   Tobias Bergmiller, Calin Guet

   Article

   Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

   Bacterial Resistance

   None

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

6. B95(DE3) ΔA ΔfabR ΔserB

   Bacterial Strain

   #197655

   Purpose

   This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

   Depositor

   Ryan Mehl, Richard Cooley

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Bacterial Resistance

   None

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

7. Ochre (rEcΔ2.ΔA.B3.tW*)

   Bacterial Strain

   #234622

   Purpose

   Genomically recoded bacteria for site-specific incorporation of two nonstandard amino acids into proteins

   Depositor

   Farren Isaacs

   Article

   Grome et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

   Bacterial Resistance

   Hygromycin

   Available Since

   May 16, 2025

   Availability

   Academic Institutions and Nonprofits only

8. C321.∆A T7RNAP ompT- rne- lon-

   Bacterial Strain

   #182778

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Spectinomycin

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

9. BL21(DE3)-R3-pRARE2

   Bacterial Strain

   #26242

   Purpose

   Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Bacterial Resistance

   Chloramphenicol

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

10. BJ5183 cells

    Bacterial Strain

    #16398

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Bacterial Resistance

    Streptomycin

    Species

    Escherichia coli

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

11. RHO3

    Bacterial Strain

    #124700

    Purpose

    E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

    Depositor

    Herbert Schweizer

    Article

    Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

    Bacterial Resistance

    Dap (400 µg/ml)

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

12. C321.∆A.opt

    Bacterial Strain

    #87359

    Purpose

    This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

    Depositor

    George Church

    Article

    Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

    Bacterial Resistance

    Gentamicin

    Species

    Synthetic

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

13. sAJM.1504

    Bacterial Strain

    #108251

    Purpose

    Marionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10B

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Bacterial Resistance

    Chloramphenicol

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

14. C321.∆A ompT- rne- endA- lon-

    Bacterial Strain

    #182775

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Ampicillin

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

15. ∆cosσε ∆HG

    Bacterial Strain

    #197132

    Purpose

    Deficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Bacterial Resistance

    Hygromycin

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

16. Syn61Δ3(ev5) ΔrecA (ev1)

    Bacterial Strain

    #189857

    Purpose

    Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

    Depositor

    George Church

    Article

    Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

    Bacterial Resistance

    Streptomycin

    Species

    Synthetic

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

17. C321.ΔA.exp

    Bacterial Strain

    #49018

    Purpose

    This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Bleocin (zeocin)

    Species

    Escherichia coli

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

18. S2060

    Bacterial Strain

    #105064

    Purpose

    host strain for phage assisted continuous evolution

    Depositor

    David Liu

    Article

    Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

    Bacterial Resistance

    Streptomycin and tetracycline

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

19. E. coli K-12 MG1655 RARE

    Bacterial Strain

    #61440

    Purpose

    This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes

    Depositor

    Kristala Prather

    Article

    Kunjapur et al J Am Chem Soc. 2014 Aug 20;136(33):11644

    Bacterial Resistance

    None

    Available Since

    Feb. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

20. Escherichia coli DH10B-MOB

    Bacterial Strain

    #229395

    Purpose

    Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Bacterial Resistance

    60 ug/ml of dap

    Species

    Escherichia coli

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only