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27,467 results

Showing: 8081 - 8100 of 27467 results

  1. pcDNA3.1-GGamma12-GFP2

    Plasmid
    #166781
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma12-GFP2 (GNG12 Synthetic, Human)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    166781-plasmid-map-sequence-id-331013

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  7. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

    Plasmid
    #137830
    Purpose
    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor
    Depositor
    Joshua Leonard
    Publication
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Mutated Leucine 71 to Lysine in CTEVp fragment
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137830-plasmid-map-sequence-id-311956

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  8. SpyTag-RBD

    Plasmid
    #159999
    Purpose
    Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells
    Depositor
    Mark Howarth
    Publication
    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159999-plasmid-map-sequence-id-313235

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  9. mMaple-OMP25

    Plasmid
    #141151
    Purpose
    Photoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.
    Depositor
    Gia Voeltz
    Publication
    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OMP25 (Synj2bp Rat)
    Tags
    mMaple
    Expression
    Mammalian
    Mutation
    OMP25 c terminus from addgene plasmid #69598
    Promoter
    CMV
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    141151-plasmid-map-sequence-id-309904

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  11. pcDNA3.1.Gα11-LgB97

    Plasmid
    #134361
    Purpose
    Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gα11-LgB97 (GNA11 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134361-plasmid-map-sequence-id-306399

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  12. opto-Rac1

    Plasmid
    #135396
    Purpose
    Expresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in mammalian cells in the pcDNA3.1 backbone.
    Depositor
    Brian Chow
    Publication
    Berlew et al Photochem Photobiol Sci. 2020 Mar 1;19(3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    opto-Rac1
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135396-plasmid-map-sequence-id-271132

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Showing: 8081 - 8100 of 27467 results