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27,467 results

Showing: 8701 - 8720 of 27467 results
  1. gag-TadCBEd

    Plasmid
    #225144
    Purpose
    Packaging plasmid for TadCBEd in v3 PE-eVLP architecture
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLVgag-TadCBEd
    Expression
    Mammalian
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pDN-Sth1Cas9

    Plasmid
    #221386
    Purpose
    Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sth1 Cas9
    Use
    CRISPR
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pCXLE-Sox2-17-T2A-Klf4-E2A-Myc

    Plasmid
    #210016
    Purpose
    episomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for integration-free naïve reset
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
    Expression
    Mammalian
    Mutation
    Sox2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

    Plasmid
    #209080
    Purpose
    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    A53T
    Promoter
    TRE4G
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  7. LD-GA

    Plasmid
    #209862
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddGFP A
    Tags
    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. 4X PRE TK luc

    Plasmid
    #206159
    Purpose
    Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    4X PRE TK luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Available since
    Nov. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  9. Salsa-BC1-P1A1

    Plasmid
    #202842
    Purpose
    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP/BC1
    Expression
    Mammalian
    Promoter
    Tet Response Element (TRE)
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pS648∙CsR

    Plasmid
    #197860
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA scaffold - Gm resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pYS17

    Plasmid
    #202083
    Purpose
    Parent vector for producing universal minicircle donors for mNeonGreen N-terminal knock-in
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NT PuroR-T2A-mNG Minicircle Parent
    Use
    CRISPR
    Tags
    mNeonGreen
    Promoter
    None
    Available since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 8701 - 8720 of 27467 results