27,467 results
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Plasmid#225144PurposePackaging plasmid for TadCBEd in v3 PE-eVLP architectureDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMMLVgag-TadCBEd
ExpressionMammalianAvailable sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN-Sth1Cas9
Plasmid#221386PurposeCas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attBDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSth1 Cas9
UseCRISPRAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
P3-dCas9-Tet1-GFP-Puro
Plasmid#190728PurposeExpression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-tet1
UseCRISPRTags3XFLAG and SV40 NLSExpressionMammalianMutationChanged Pro434 codon from CCG to CCC and Gly468 f…PromoterpCAGAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-µLight
Plasmid#201225PurposeExpress muLight biosensor in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertµLight
ExpressionMammalianPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK EGFP
Plasmid#216110PurposePGK EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPGK promoter
TagsEGFPExpressionMammalianPromoterPGKAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gTBEv5
Plasmid#220621Purposevector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
ExpressionMammalianMutationhUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…PromoterhU6, EF1aAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
modRNAc1-VP64dCas9VP64-2A-MPH
Plasmid#216388Purposethis vector can be used to make modRNA expressing VP64dCas9VP64 and MPHDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVP64dCas9VP64-2A-MPH
UseCRISPRAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-hSTING (N154S)
Plasmid#214160PurposeTransient expression of gain-of-function STING mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSTING (N154S) (STING1 Human)
ExpressionMammalianMutationN154SPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
qMaLioffG
Plasmid#212825Purposegreen fluorescent FLIM indicator for endogenous ATP imagingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertqMaLioffG
ExpressionMammalianPromoterCMVAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-Sox2-17-T2A-Klf4-E2A-Myc
Plasmid#210016Purposeepisomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for integration-free naïve resetDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianMutationSox2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)
Plasmid#209080PurposeNgn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagssfGFPExpressionMammalianMutationA53TPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LD-GA
Plasmid#209862PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddGFP A
TagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
4X PRE TK luc
Plasmid#206159PurposeLuciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert4X PRE TK luciferase
UseLuciferaseExpressionMammalianAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Salsa-BC1-P1A1
Plasmid#202842PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP/BC1
ExpressionMammalianPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS648∙CsR
Plasmid#197860PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYS17
Plasmid#202083PurposeParent vector for producing universal minicircle donors for mNeonGreen N-terminal knock-inDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNT PuroR-T2A-mNG Minicircle Parent
UseCRISPRTagsmNeonGreenPromoterNoneAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hMC4R
Plasmid#179315PurposeExpress human melanocortin 4 receptor (GFP tagged) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmelanocortin 4 receptor (MC4R Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Integrin β4-GFP
Plasmid#205092PurposeExpress GFP-tagged human integrin β4 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIntegrin beta 4 (ITGB4 Human)
TagsGFPExpressionMammalianAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mCherry-RUSH
Plasmid#202799PurposeRUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLamp1 (Lamp1 Rat)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)
Plasmid#203908PurposeEncodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGal4UAS_dGFP_PGK_iRFP
ExpressionMammalianPromoter5xUAS and PGKAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only