27,467 results
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Plasmid#122509PurposeCombining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-GCN5
UseCRISPRTags3*FLAGMutationdCas9(D10A,H840A)PromoterPfHsp86Available sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-RAB-EKARev
Plasmid#118477PurposeddRFP-based single-color ERK biosensor targeted to the plasma membrane.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn-RAB-EKARev
TagsN-terminal targeting sequence from Lyn-kinaseExpressionMammalianPromoterCMVAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV GFP-LC3B G120A
Plasmid#123115PurposeExpresses EGFP-LC3B G120A in mammalian cells. Negative control fluorescent reporter, unable to localize to autophagosomes.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
TagsEGFPExpressionMammalianMutationGlycine 120 to AlaninePromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Orange_CaMBI_110
Plasmid#124094Purposeorange calcium-modulated bioluminescent indicator, 110nMDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOrange CaMBI 110
ExpressionMammalianAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
Plasmid#119946PurposeExpresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRenilla luciferase
Tags5'TOP sequence, 24xGCN4 repeats (SunTag), 24…ExpressionBacterial and MammalianPromoterTetCMVAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTag-NLS-eGFP-SV40
Plasmid#117811PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UseSynthetic BiologyTagsNLSAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTag-eGFP-CAAX-SV40
Plasmid#117815PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UseSynthetic BiologyTagsCAAXAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-TagRFP-SV40
Plasmid#117805PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTagRFP
UseSynthetic BiologyTagsNLSAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM4787
Plasmid#120088PurposeCyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPlasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
UseOtherAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cmv d0 dPspCas13b-GGS-NYTHDF1
Plasmid#119855PurposeCmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas13b-GGS-NYTHDF1
UseCRISPRExpressionMammalianAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-blueCKAR
Plasmid#118472PurposecpBFP-based single-color biosensor for monitoring Protein Kinase C activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertblueCKAR
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-blueAKAR
Plasmid#118470PurposecpBFP-based single-color biosensor for monitoring Protein Kinase A activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertblueAKAR
ExpressionMammalianPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Snapin
Plasmid#118742PurposeExpresses EGFP tagged SnapinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSnapin cDNA (Snapin Mouse)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCR3.1-Luc
Plasmid#106457Purposeluciferase reporter, CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertluciferase reporter, CMV promoter
UseLuciferaseExpressionMammalianMutationWT promoterAvailable sinceNov. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS mSynCAM1
Plasmid#115496Purposeexpression of mouse full-length SynCAM 1, CAG promoter suited for neuronal expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCadm1 (Cadm1 Mouse)
ExpressionMammalianPromoterchicken beta-actinAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Rev(NL4.3)
Plasmid#115776PurposepCMV-RevNL4.3 allows the expression of Rev protein.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHIV-1 Rev protein (NL4.3)
ExpressionMammalianMutationNonePromoterCMVAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Kv7.4 (1-695)/pcDNA3.1
Plasmid#111453PurposeElectrophysiology in HEK cellDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
Tags8xHis and EGFPExpressionMammalianMutationKv7.4 (1-695)Available sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-PKCb1-mEGFP-N1
Plasmid#112265PurposeITRACKbeta/IDOCKSbeta Donor constructDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertprotein kinase c beta (Prkcb Mouse)
TagsmEGFPExpressionMammalianPromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-Rab7(WT)
Plasmid#112959PurposeIn vivo visualization of Rab7 (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab7
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEBTet_NAD-Snifit-nucl
Plasmid#113915PurposePlasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNAD-Snifit
ExpressionMammalianPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only