27,467 results
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Plasmid#154260PurposeDestabilized firefly luciferase reporter containing the upstream Human PGC-1alpha Distal Promoter from -500 to +52.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPGC-1a Distal Promoter (PPARGC1A Human)
ExpressionMammalianPromoterHuman PGC-1alpha Distal PromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC1-CMV-DAAO-NES
Plasmid#141107PurposeExpresses DAAO with nuclear export signal in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDAAO-NES
TagsNuclear export signalExpressionMammalianMutationdeleted DAAO amino acids 367-368PromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
hyA3A-BE4max
Plasmid#157943PurposeMammalian expression of hyA3A-BE4maxDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthyA3A-BE4max
UseCRISPRExpressionMammalianAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCD5-H3TX12-CO-GCN4-TEV-sfGFP-TS
Plasmid#158739PurposeExpresses influenza A virus trimeric hemagglutinin A/H3/TX/12 fused to a sfGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHemagglutinin A/TX/50/12 H3
TagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationG186V S219FPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BRD4-N CRISPR
Plasmid#140651PurposeBRD4 tagging CRISPRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBRD4 (BRD4 Human)
UseCRISPRExpressionMammalianAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAG364-greenFAST
Plasmid#160353Purposeexpresses greenFAST in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgreenFAST
ExpressionMammalianAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector
Plasmid#159281PurposeA multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAsCpf1-HA-2A-GFP
UseMulticistronic vector with both caggs promoter-dr…Tags3X HAExpressionMammalianPromoterU6Available sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor
Plasmid#156432PurposeVector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCTCF, rtta3G, TetO3G (Ctcf Mouse)
UseMouse TargetingExpressionMammalianMutationnoneAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
B-MFF
Plasmid#141158PurposeDimerization-dependent fluorescent protein binding partner targeted to outer mitochondrial membraneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmitochondrial fission factor (MFF Human)
TagsGBExpressionMammalianPromoterCMVAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee-NES
Plasmid#140901Purposemammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signalingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpiCee-NES
TagsmRFPExpressionMammalianPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCX-NTOM-SpiCee
Plasmid#140902Purposemammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signalingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNTOM-SpiCee
TagsmRFPExpressionMammalianPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RTP1S
Plasmid#149364PurposeExpresses RTP1S in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRTP1S (Rtp1 Mouse)
ExpressionMammalianPromoterCMVAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE-SHH
Plasmid#134350PurposeTargeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSHH (SHH Human)
ExpressionMammalianAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CBh-dCas9-hUtx-IRES-Hyg
Plasmid#122374PurposeExpresses dCas9-hUtx-IRES-Hyg in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCBh-dCas9-hUtx-IRES-Hyg-pA
UsePiggybacExpressionMammalianAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133747
Plasmid#152494PurposeMammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHis-003ExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA4/TO/GFP-TTP
Plasmid#141190PurposeExpresses GFP-TTP in mammalian cells, can be used to make inducible cell lineDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTTP (ZFP36 Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phR-Luc
Plasmid#139644PurposeMammalian gene expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRenilla luciferase
UseLuciferasePromoterSV40Available sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4-SsAPOBEC3B
Plasmid#138343PurposeMammalian expression plasmid for BE4-SsAPOBEC3BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE4-SsAPOBEC3B
UseSynthetic BiologyTagsBP-NLSPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Akap1(31)-FRB-iRFP
Plasmid#139315Purposemitochondrial-resident FRB for chemically induced dimerizationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMus musculus Akap1(1-31) M16L:MTOR(2021-2113):iRFP
ExpressionMammalianMutationM16LAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)
Plasmid#139993PurposeCMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationxCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only