27,466 results
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Plasmid#115365PurposeExpression vector to produce humanized Renilla luciferase with 5BoxB sequences in the 3'UTRDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRenilla Luciferase
UseLuciferaseExpressionMammalianPromoterTKAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eGFP-pk-S65A
Plasmid#115619PurposeExpresses human Parkin S65A in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertParkin (PRKN Human)
TagsEGFPExpressionMammalianMutationS65APromoterCMVAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cdt1 human
Plasmid#72681Purposemammalian expression of human Cdt1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCdt1 (CDT1 Human)
ExpressionMammalianPromoterCMVAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q48
Plasmid#111747PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ48 (polyQ repeat)PromoterCMV and P10Available sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
BK beta2 EGFP
Plasmid#113558Purposehuman BK beta2 subunit in C-term translational frame with EGFP (EGFP-N1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKcnmb2 (KCNMB2 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRE-T2-IRES-His6-Ubiquitin
Plasmid#107107PurposeDesigned to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman ubiquitin UBB (UBB Human)
Tags6xHisExpressionMammalianPromoterTetAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gata4-GFP donor
Plasmid#113115PurposeDonor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGata4 donor region including homology arms and eGFP
UseSynthetic BiologyAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_eGFP_497
Plasmid#111824PurposeKnockout eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA targeting eGFP 477-497
UseCRISPRTagsmCherryExpressionBacterial and MammalianPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-NES-ZapCV2 (cpV143)
Plasmid#112060PurposeGenetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNES-ZapCV2
TagsNuclear Export Signal (NES) derived from HIV-1ExpressionMammalianMutationECFP gene is truncated 36 bp at 3' end. Venu…PromoterCMVAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRPaCas9
Plasmid#111819PurposeExpresses Cas9 in T. brucei (2T1) cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
UseCRISPRAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
tCRMod-GGSGGS7
Plasmid#111761PurposeExpresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClover-GGSGGS7-mRuby2 fusion
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS6-StaPLd-Casp9
Plasmid#111511PurposeExpresses a tandem caspase-9 dimer, governed by a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStaPLd-Casp9
ExpressionMammalianMutationThe HCV NS3 protease carries V36M, T54A, and S122…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry Codon 59 sgRNA
Plasmid#109431PurposeMLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry codon 59 gRNA
UseCRISPRExpressionMammalianPromoterhU6Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1-EXO1b-D173A
Plasmid#111622PurposeExpression of human catalitic dead Exonuclease 1 (EXO1) mCherry-tagged in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertExonuclease 1b D173A (EXO1 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mVenusC-Cavin1
Plasmid#108888PurposeExpression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCavin1 (altered codon usage) (CAVIN1 Human, Synthetic)
TagsmVenus156-239ExpressionMammalianMutationaltered codon usageAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChR2-mRuby2-ST
Plasmid#109125PurposeCation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertChR2-mRuby2-ST
ExpressionMammalianPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mVenusN-Cavin1
Plasmid#108887PurposeExpression of Cavin1 fused to the N-terminus of mVenus for bimolecular fluorescence complementationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcaveolae associated protein 1 (CAVIN1 Human, Synthetic)
TagsmVenus1-155ExpressionMammalianMutationaltered codon usageAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluu
Plasmid#105832PurposeFast indicator for extracellular glutamate imagingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiGluSnFR S72T variant
TagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (1-517, C77A, MG-38-16)
Plasmid#110749PurposeTransient transfection of human USP30 C77ADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUSP30 (USP30 Human)
ExpressionMammalianMutationC77APromoterCMVAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_LMNA
Plasmid#111688PurposeMAC-tagged gene expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNA (LMNA Human)
ExpressionMammalianAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only