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27,466 results

Showing: 11861 - 11880 of 27466 results

  3. pMax_mOrange_A215C

    Plasmid
    #177828
    Purpose
    Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).
    Depositor
    Zachary Nagel, Leona Samson
    Publication
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mOrange_A215C
    Expression
    Mammalian
    Mutation
    A215C
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177828-plasmid-map-sequence-id-348949

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  4. pSems-leader-mXFPm-IFNGR1(18-489)

    Plasmid
    #192786
    Purpose
    Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Publication
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFNGR1 (IFNGR1 Human)
    Tags
    Ig k-chain leader sequence and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192786-plasmid-map-sequence-id-379664

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  5. pLVX-hMIGA2-DsRed

    Plasmid
    #192866
    Purpose
    Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells
    Depositor
    Karin Reinisch
    Publication
    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    Tags
    DsRed
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192866-plasmid-map-sequence-id-379959

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  7. plk1 T210A

    Plasmid
    #132965
    Purpose
    To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage
    Depositor
    Michael Yaffe
    Publication
    Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    polo-like kinase 1 T210A (PLK1 Human)
    Expression
    Mammalian
    Mutation
    T210A
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    132965-plasmid-map-sequence-id-265935

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  10. pcDNA3.1-3xhfYFP

    Plasmid
    #186544
    Purpose
    Hyperfolder YFP, triple fusion: cytosolic expression in mammalian cells
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hyperfolder YFP (3X)
    Expression
    Mammalian
    Mutation
    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186544-plasmid-map-sequence-id-368234

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  11. LifeAct-LSSmGFP

    Plasmid
    #186534
    Purpose
    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LSSmGFP
    Tags
    LifeAct
    Expression
    Mammalian
    Mutation
    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186534-plasmid-map-sequence-id-368226

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  12. Tubulin-hfYFP

    Plasmid
    #186515
    Purpose
    Localization of hyperfolder YFP fluorescent protein to tubulin
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hyperfolder YFP
    Tags
    Tubulin
    Expression
    Mammalian
    Mutation
    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186515-plasmid-map-sequence-id-368091

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  14. pLenti-PGK-EGFP-ALDH1A2-Neo

    Plasmid
    #189743
    Purpose
    The construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.
    Depositor
    Robert Sobol
    Publication
    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ALDH1A2 (ALDH1A2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    189743-plasmid-map-sequence-id-374852

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  15. pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

    Plasmid
    #187953
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-SpdCas9-KRAB-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187953-plasmid-map-sequence-id-373487

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  16. pBS L30 mRuby3-Rab18

    Plasmid
    #166431
    Purpose
    Rab18 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Raphael Gaudin
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab18 (RAB18 Human)
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    L30 weak promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166431-plasmid-map-sequence-id-333545

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  19. pSBbi_RP_MicroID2-FLAG-NES

    Plasmid
    #188525
    Purpose
    Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Publication
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MicroID2
    Use
    Transposon mediated gene integration
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188525-plasmid-map-sequence-id-372837

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  20. pSBbi_RP_3XNLS-MicroID2-FLAG

    Plasmid
    #188523
    Purpose
    Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Publication
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3XNLS-MicroID2
    Use
    Transposon mediated gene integration
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188523-plasmid-map-sequence-id-372834

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Showing: 11861 - 11880 of 27466 results