27,006 results
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Plasmid#89448PurposeExpression of GFP-tagged human Rab27B Q78L in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman Rab27B Q78L (RAB27B Human)
TagseGFPExpressionMammalianMutationQ78LPromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTH2
Plasmid#84452Purposeshuttle plasmid for sarA P1-CFP expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCFP Cerulean
UseShuttle vector e.coli-s.aureus, expression of sgf…Available sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
J23104-mRFP1-331Bb
Plasmid#78274PurposemRFP1 behind constitutive promoter J23104 in pSEVA331Bb backboneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertJ23104-mRFP1
Available sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DT480
Plasmid#80413PurposeExpresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
ExpressionMammalianAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Puro-iNotch1IC
Plasmid#75338Purposedoxycycline inducible Notch1 intracellular domain gene over-expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNotch1 Intracellular domain (Notch1 Mouse)
ExpressionMammalianPromoterTRE-tightAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6xO/S luc
Plasmid#69445Purposedetection/assessment of OCT4 and SOX2 transcription factor activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertminimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
UseLuciferaseExpressionMammalianAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX462-hPRKAA1-gRNA_B
Plasmid#74375PurposegRNA_B to knockout human AMPK alpha 1 using Cas9nDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
UseCRISPRPromoterU6Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK281 (mCherry2-Hygro)
Plasmid#72797PurposeC-terminal tagging with mCherry2 using CRIPSR/Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry2-Hygro
UseCRISPRTagsmCherry2ExpressionMammalianAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
G-GECO1.2-Orai1
Plasmid#73562PurposeGreen fluorescent reporter of Orai1-associated calcium influxDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertG-GECO1.2-Orai1 (ORAI1 Synthetic, Human)
TagsG-GECO1.2ExpressionMammalianPromoterCMV Immediate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(-) FLIPQTV3.0 100micro
Plasmid#63730PurposeFLIPQTV3.0 glutamine sensor with D157N mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLIPQTV3.0 sensor, 100microM affinity
ExpressionMammalianMutationD157N in glutamine binding domainAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCer-C3-Plk1-K82R
Plasmid#68134PurposeExpression of Cerulean-tagged, kinase-dead Plk1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPolo-like kinase 1 (PLK1 Human)
TagsCeruleanExpressionMammalianMutationK82R kinase-dead versionPromoterCMVAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ch-TOGKDP-GFP
Plasmid#69112PurposeFor mammalian expression of knockdown-proof human ch-TOG tagged with EGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertColonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
TagsEGFPExpressionMammalianMutationSilent mutations to confer shRNA resistance.PromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6_(GLuc)_INT[BoBS]
Plasmid#68431PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertINT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
GPRC5B-Tango
Plasmid#66383PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPRC5B (GPRC5B Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GIPR-Tango
Plasmid#66294PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGIPR (GIPR Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pECFP-Sec31A
Plasmid#66612PurposeCFP fusion of Sec31ADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSec31A (SEC31A Human)
TagsECFPExpressionMammalianAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ii-Str_SBP-mCherry-GPI
Plasmid#65297Purposesynchronize trafficking of mCherry-GPI from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPI anchor
TagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianPromoterCMVAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_ManII-SBP-EGFP
Plasmid#65255Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIi-Streptavidin and truncated Mannosidase II
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mPA-GFP-Actin-7
Plasmid#57120PurposeLocalization: Actin, Excitation: 400 / 504, Emission: 515 / 517DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertActin (ACTB Human)
TagsmPA-GFPExpressionMammalianPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
PA-GFP Golgi
Plasmid#57164PurposeLocalization: Golgi, Excitation: 400 / 504, Emission: 515 / 517DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGolgi (B4GALT1 Human)
TagsPA-GFP GolgiExpressionMammalianPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by