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50 µg Transfection-Ready DNA Clear filter

27,006 results

Showing: 6581 - 6600 of 27006 results

  1. pAc5 HA3-eDHFR-T2A-puro

    Plasmid
    #86395
    Purpose
    for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection
    Depositor
    David Bentley
    Publication
    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HA3 eDHFR T2A puro
    Use
    CRISPR
    Tags
    HA3 eDHFR T2A puro
    Mutation
    R12Y, G67S and Y100I mutations in E coli DHFR
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    86395-plasmid-map-sequence-id-170910

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  3. LVXN-Neo-NSD2-E1099K

    Plasmid
    #86011
    Purpose
    express NSD2 E1099K mutant in mammalian cells
    Depositor
    Darrin Stuart
    Publication
    Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NSD2 E1099K mutant (NSD2 Human)
    Tags
    Flag tag  D Y K D D D D K
    Expression
    Mammalian
    Mutation
    E1099K; K1002R (please see depositor comments bel…
    Promoter
    CMV
    Available since
    Jan. 13, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. mCer-TG2-YFP

    Plasmid
    #85471
    Purpose
    TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET
    Depositor
    Jeffrey Keillor, Ray Truant
    Publication
    Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hTG2 (TGM2 Human)
    Tags
    EYFP and mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    85471-plasmid-map-sequence-id-165712

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  8. pMA-SpCas9-g2

    Plasmid
    #80785
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80785-plasmid-map-sequence-id-162403

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  9. DT240

    Plasmid
    #80414
    Purpose
    Expresses 240 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Publication
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
    Expression
    Mammalian
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    80414-plasmid-map-sequence-id-151401

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  13. pMaster3

    Plasmid
    #58526
    Purpose
    Reprogram somatic cells to iPS cells
    Depositor
    Mario Capecchi
    Publication
    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 )
    myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    Nanog (NANOG Human)
    IRES
    NR5A2 (NR5A2 Human)
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    58526-plasmid-map-sequence-id-90649

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  16. psiCHECK2-let-7 MT

    Plasmid
    #78261
    Purpose
    psiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Publication
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mutated let-7 target site
    Use
    Luciferase; Microrna activity
    Expression
    Mammalian
    Mutation
    nucleotides 2-5 and 10-11 changed from CTCC to GA…
    Available since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    78261-plasmid-map-sequence-id-149478

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  20. mCherry-CAD

    Plasmid
    #73566
    Purpose
    Soluble red fluorescent activator of Orai1 channels
    Depositor
    Michael Cahalan
    Publication
    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-CAD (STIM1 342-448) (STIM1 Synthetic, Human)
    Tags
    6x His and mCherry
    Expression
    Mammalian
    Promoter
    CMV Immediate Early
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    73566-plasmid-map-sequence-id-137397

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Showing: 6581 - 6600 of 27006 results