27,006 results
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Plasmid#86596PurposeExpresses mCherry-tagged PH(WT) domain of human Akt2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAkt2 PH domain (AKT2 Human)
ExpressionMammalianMutationAmino acids 1 - 108Available sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLI-2xFKBP-GFP(1463000)
Plasmid#85789PurposeP. falciparum integration plasmid to fuse endogenous genes with tags using SLIDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R
UseP. falciparum integrationTags2xFKBP, GFPPromoternoneAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC+] (AVA2626)
Plasmid#85444PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9
Plasmid#84246PurposeExpresses direct fusion VPR-Sa dCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsVPR-tagBFP-Sa dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTagsNucleoplasmin NLS, SV40 NLS, VPR, and tagBFPExpressionMammalianPromoterCAG and PGKAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1-Pur-CAG-hM4Di-mCherry
Plasmid#80947Purposehuman AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthM4Di-mCherry
Tagsmcherry fusionExpressionMammalianPromoterCAG promoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9
Plasmid#84243PurposeExpresses ABA-inducible VPR-Sa dCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsABI-tagBFP-Sa dCas9
PYL1-VPR
UseCRISPR and Synthetic Biology; PiggybacTagsABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …ExpressionMammalianMutationD10A, N580A and Synonymous mutation in PYL1 to re…PromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-T31N (DN)
Plasmid#83423PurposeMouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab21 (Rab21 Mouse)
TagsEGFPExpressionMammalianMutationResidue 31 from T to N (T31N: ACG -> AAC)PromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTol2p2A--3mnx1:mTagBFP-NS-MCS
Plasmid#78250PurposeCan be used to subclone in gene of interest into blue motor neuron expression vector.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseZebrafish motor neuron expressionPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPaint-TagBFP-PuroR
Plasmid#80969PurposeCRISPaint gene taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTagBFP
UseGene taggingPromoternoneAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB1deltaC-mCherry
Plasmid#80603PurposeExpresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus endDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEB1 (MAPRE1 Human)
TagsmCherryExpressionMammalianMutationTruncated C-terminus; EB1 coding sequence ends at…PromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmCherry-Paxillin
Plasmid#80029PurposeMammalian expression of mouse PaxillinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPaxillin (Pxn Mouse)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmiRFP703-Clathrin
Plasmid#79997PurposeClathrin labeling with near-infrared fluorescent protein miRFP703DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClathrin (CLTB Human)
TagsmiRFP703ExpressionMammalianPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLCN-eGFP
Plasmid#72289PurposeExpresses human FLCN tagged with eGFP at C terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLCN (FLCN Human)
TagseGFPExpressionMammalianAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-INPP5E
Plasmid#79561Purposeexpression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motifDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertINPP5E (INPP5E Human)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPR220.08
Plasmid#78553PurposePlasmid constitutively expressing phycocyanobilin (PCB) biosynthesis genes and UirS sensor kinase geneDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertuirS
UseSynthetic BiologyPromoterJ23108Available sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA2-gRNA_B
Plasmid#74377PurposegRNA_B to knockout human AMPK alpha 2 using Cas9nDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
UseCRISPRPromoterU6Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK280 (mCherry2-NeoR)
Plasmid#72796PurposeC-terminal tagging with mCherry2 using CRIPSR/Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry2-Bsr
UseCRISPRTagsmCherry2ExpressionMammalianAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-C1483F
Plasmid#69008Purposeactivating MTOR mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMTOR-C1483F (MTOR Human)
TagsFLAGExpressionMammalianMutationchange Cysteine 1483 to PhenylalanineAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Nanog-2A-mCherry
Plasmid#48680PurposeFor knock-in donor vector by CRISPR/CAS systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNanog (Nanog Mouse)
UseCRISPRTagsCherryAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
sgPal7 (p2Tol-U6-sgPal7-HygR)
Plasmid#71484PurposeExpresses palindromic sgRNA for use in self-cloning CRISPRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgPal7
PromoterU6Available sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by