27,006 results
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Plasmid#91036PurposeModule A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCsy4-P2A-TaCas9
UseCRISPRPromoterZmUbiAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CRY2low-tdTomato
Plasmid#104067PurposeExpresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomatoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRY2low-tdTomato
ExpressionMammalianMutationCRY2 aa 1-491 truncation, R489E A491DPromoterCMVAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNup133-Ypet
Plasmid#98283Purposemammalian expression of Nup133-YpetDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNup133 (NUP133 Human)
TagsYPetExpressionMammalianPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Kif5A-CIBN
Plasmid#102251PurposeExpresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
px459 VRER
Plasmid#101716PurposesgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 VRER
Tags3xFLAG-NLS and NLSExpressionMammalianMutationVRER (D1135V, G1218R, R1335E and T1337R)Available sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOTTC592 - pCMV Mito-iRFP
Plasmid#59135PurposeA plasmid that express mitochondrial-targeted iRFP under the CMV promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMitochondria-localized Infrared Fluorescent Protein
TagsMitochondria-targeting sequenceExpressionMammalianPromoterCMV-IEAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCX-H2B-KikGR
Plasmid#98649PurposeExpresses a nuclear-localized photoconvertible green-to-red fluorescent proteinDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKikGR
TagsH2BExpressionMammalianPromoterCMV-IE enhancer chick beta actin promoterAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-NEAT1pr_v2
Plasmid#97082PurposeEncodes an sgRNA that creates a DSB at the promoter of human NEAT1 geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA NEAT1pr_v2
UseCRISPRPromoterU6Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CIB-VP64 (B1016)
Plasmid#92037PurposeExpresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCIB1-VP64 (CIB1 Mustard Weed)
TagsFusion of plant CIB1 with VP64 activation domain.…ExpressionMammalianPromoterCMVAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CRY-Gal∆DD (B1013)
Plasmid#92035PurposeExpresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRESDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRY2 (CRY2 Mustard Weed)
ExpressionMammalianMutationFusion of plant CRY2 to Gal4 binding domain (AA1-…Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)
Plasmid#92400PurposeConstitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and membrane tagAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2515
Plasmid#91083PurposeModule C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRPromoterAtU6Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Px461-Cas9n-Trp53-sgRNA-beta
Plasmid#88847PurposeCRISPR KO of Trp53DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTrp53 (Trp53 Mouse)
UseCRISPRExpressionMammalianAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-IRAlus-Lin28
Plasmid#92347PurposePlasmid for expression of GFP gene with a Lin28 gene derived IR Alu element containing 3'UTR.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLin28 gene derived IR Alu element containing 3'UTR (LIN28A Human)
ExpressionMammalianPromoterCMVAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-Otx2-IRES-GFPd2
Plasmid#73996PurposePlasmid expressing Otx2(isoform b)-IRES-GFPd2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOtx2(isoform b)-Ires-GFP (Otx2 Mouse)
ExpressionMammalianPromoterCAGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-C12orf66
Plasmid#87047Purposemammalian expression of C12orf66DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertC12orf66 (C12orf66 Human)
TagsFlagExpressionMammalianPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLyn-FRB-mCherry-nmd3-BSD
Plasmid#85796PurposeExpression of PPM mislocaliser in P. falciparumDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPPM Mislocaliser
UseP. falciparum expressionTagsFRB*, mCherryPromoterPfnmd3Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-AMPH1-BAR-mCherry
Plasmid#85130PurposeBAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)
TagsmCherryExpressionMammalianPromoterCAGAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Fireworks GFP[PTC+] (AVA2600)
Plasmid#85446PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgTP53(A)
Plasmid#85559PurposeExpresses sgRNA targeting human TP53 around codon F109DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttumor protein p53 (TP53 Human)
ExpressionMammalianAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by