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27,462 results

Showing: 9041 - 9060 of 27462 results

  1. pS548∙CsR

    Plasmid
    #197859
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Publication
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA scaffold - Tc resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197859-plasmid-map-sequence-id-393256

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  2. pS648∙CsR

    Plasmid
    #197860
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Publication
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA scaffold - Gm resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197860-plasmid-map-sequence-id-402537

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  4. pJAT31

    Plasmid
    #204291
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ie1-DsRed
    Use
    CRISPR
    Promoter
    ie1
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204291-plasmid-map-sequence-id-405841

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  5. pJAT17

    Plasmid
    #204298
    Purpose
    For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3XP3-EYFP
    Use
    CRISPR
    Promoter
    3XP3
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204298-plasmid-map-sequence-id-406176

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  11. pcDNA3.1-PDGFRA-V5/HIS

    Plasmid
    #201987
    Purpose
    expression of human PDGFRA receptor tyrosine kinase in mammalian cells
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PDGFRA receptor tyrosine kinase (PDGFRA Human)
    Tags
    V5, 6xHis
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    201987-plasmid-map-sequence-id-396816

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  12. pAf-CRISPR-yA

    Plasmid
    #191015
    Purpose
    CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.
    Depositor
    Perng-Kuang Chang
    Publication
    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    yA
    Use
    CRISPR
    Promoter
    Aspergillus flavus U6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    191015-plasmid-map-sequence-id-516753

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  13. pCI pHluorin Syt1

    Plasmid
    #184502
    Purpose
    Mammalian expression of Synaptotagmin 1 with pH-sensitive fluorescent protein
    Depositor
    James Rothman
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Synaptotagmin 1 (Syt1 Rat)
    Tags
    Super Ecliptic pHluorin
    Expression
    Mammalian
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    184502-plasmid-map-sequence-id-393122

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  15. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197027
    Purpose
    Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized enRhCas12f1
    Expression
    Mammalian
    Mutation
    L270R
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197027-plasmid-map-sequence-id-388964

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  16. pcmv3-IRG1-HA

    Plasmid
    #198181
    Purpose
    Mammalian expression of HA-tagged human ACOD1 (IRG1)
    Depositor
    Yun Zhao
    Publication
    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACOD1 (ACOD1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    HA tag is added to C-terminal of target protein
    Promoter
    CMV
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198181-plasmid-map-sequence-id-389087

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  18. pN7CAST

    Plasmid
    #190661
    Purpose
    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190661-plasmid-map-sequence-id-385667

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  19. pFB2F

    Plasmid
    #195289
    Purpose
    Bicistronic HSV-TK and mScarlet resistance for positive/negative selection
    Depositor
    Scott Lowe
    Publication
    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HSV-TK 2A mScarlet
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    195289-plasmid-map-sequence-id-382900

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  20. pCMV-APOBEC1-YTHD422N

    Plasmid
    #194432
    Purpose
    APOBEC1 fused to YTHD422N
    Depositor
    Kate Meyer
    Publication
    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    APOBEC1-YTHD422N (APOBEC1 Human)
    Use
    CRISPR
    Tags
    APOBEC1-YTHD422N-HA
    Expression
    Mammalian
    Mutation
    D422N mutation in the YTH domain to enhance m6A b…
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194432-plasmid-map-sequence-id-382432

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Showing: 9041 - 9060 of 27462 results